芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β<sub>1</sub>及Smad2/3的影响

高湲; 陈桂敏; 梁振钰; 谢毅强

doi:10.13422/j.cnki.syfjx.2012.15.075

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芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β₁及Smad2/3的影响

药理 | 更新时间：2024-09-23

- 芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β₁及Smad2/3的影响
- Effect of Invigorating Blood Circulation Group of Qigong Kangxian Prescription on TGF-β₁ and Smad2/3 in Liver Fibrosis Rats
- 中国实验方剂学杂志 2012年18卷第15期页码：260-262
- 作者机构：
  
  海南医学院
- 作者简介：
- 基金信息：
  
  2008年海南省自然科学基金项目（808165）
- DOI：10.13422/j.cnki.syfjx.2012.15.075
  中图分类号： R285.5
- 纸质出版日期：2012
- 稿件说明：
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[1]高湲,陈桂敏,梁振钰,谢毅强.芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β_1及Smad2/3的影响[J].中国实验方剂学杂志,2012,18(15):260-262.

GAO Yuan, CHEN Gui-min, LIANG Zhen-yu, et al. Effect of Invigorating Blood Circulation Group of Qigong Kangxian Prescription on TGF-β₁ and Smad2/3 in Liver Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 260-262.
[1]高湲,陈桂敏,梁振钰,谢毅强.芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β_1及Smad2/3的影响[J].中国实验方剂学杂志,2012,18(15):260-262. DOI： 10.13422/j.cnki.syfjx.2012.15.075.

GAO Yuan, CHEN Gui-min, LIANG Zhen-yu, et al. Effect of Invigorating Blood Circulation Group of Qigong Kangxian Prescription on TGF-β₁ and Smad2/3 in Liver Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 260-262. DOI： 10.13422/j.cnki.syfjx.2012.15.075.

摘要

目的:观察芪蚣抗纤方（QF）活血组对免疫损伤性肝纤维化大鼠转化生长因子（TGF-β1）及Smad2/3的影响。方法:猪血清腹腔注射法复制免疫损伤性肝纤维化大鼠模型

分为正常对照组

模型组、活血低剂量组、活血高剂量组

用双抗夹心法（ELISA法）测定血清TGF-β1含量;免疫组化法检测肝组织TGF-β1

Smad2

Smad3的表达。结果:①血清学指标:模型组血清TGF-β1含量较其他组明显升高（P<0.01）。与模型组比较

活血组血清TGF-β1含量显著下降（P<0.01）

并具量效关系。②免疫组化检测:模型组肝组织TGF-β1

Smad2

Smad3表达明显

活血组肝组织中TGF-β1

Smad2

Smad3表达明显低于模型组（P<0.01）。结论:芪蚣抗纤方活血组通过降低TGF-β1

减少Smad2/3的激活

从而抑制肝纤维结缔组织增生

减轻肝损伤及肝纤维化程度。具有抑制肝纤维化的作用。

Abstract

Objective: To observe the effect of invigorating blood circulation group of Qigong Kangxian prescription（QF） on transforming growth factor betal（TGF-β1） and Smad 2/3 in immunity-damaged liver fibrosis rats.Method: Use porcine serum by intraperitoneal injection to copy the rat model of immunity-damaged liver fibrosis was established by intraperitoneal injection of porcine serum rat.Use the double antibody sandwich method was used to determine the contents of serum TGF-β1.Use the immunohistochemical method was used to detect the expression of liver tissue TGF-β1

Smad2 and Smad3.Result: ①Serological index: the content of TGF-β1 in model group was significantly higher than other groups（P<0.01）.Compared with model group

the content of TGF-β1 in invigorate blood circulation group was decreased significantly（P<0.01）.And with thea manner of dose-effect relationship.②Test the results of immunohistochemical assay: the expression of TGF-β1

Smad2 and Smad3 in model was obvious.Expression of TGF-β1

Smad2 and Smad3 in invigorating blood circulation group was significantly lower than that in model group（P<0.01）.Conclusion: Invigorating blood circulation group of Qigong Kangxian prescription has the function of inhibiting liver fibrosis by inhibiting TGF-β1and Smad 2/3.

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芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β1及Smad2/3的影响

Effect of Invigorating Blood Circulation Group of Qigong Kangxian Prescription on TGF-β1 and Smad2/3 in Liver Fibrosis Rats

DOI：10.13422/j.cnki.syfjx.2012.15.075

摘要

Abstract

关键词

Keywords

references

芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β₁及Smad2/3的影响

Effect of Invigorating Blood Circulation Group of Qigong Kangxian Prescription on TGF-β₁ and Smad2/3 in Liver Fibrosis Rats