Objective:To explore the effects of Huazhuo Jiedu Recipe（HZJD） on the proliferation and activity in rat hepatic Stellate cells（HSCs） in vitro.Method:The drug-containing serum of HZJD was prepared by feeding healthy SD rats at various doses（20

15

10 mL·kg-1·d-1） of HZJD

and used to incubate the HSCs cultured in vitro.The impact of drug-containing serum on HSCs proliferation was estimated by methyl thiazolyl tetrazolium（MTT）at 24

48 h and 72 h after by intervention of drug-containing serum;the content of collagen 1（COL I） was detected by enzyme linked immunosorbent assay（ELISA）;the expression of transforming growth factor betal（TGF-β1） mRNA was measured by RT-PCR.Result: The proliferation of HSCs was inhibited significantly after intervention with various dose of HZJD drug-containing serum.With a concentration-dependent mannercompared with the normal serum control group（P<0.05）.The content of COL I and the expression of TGF-β1 mRNA were degraded after intervened with HZJD drug-containing serum at various dose in HSCs（P<0.05）.Conclusion: Drug-congtaining serum of HZJD could inhibit the proliferation and activity of HSCs in vitro.