导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响

陈文强; 黄小波; 李宗信; 王芬; 王宁群

doi:10.13422/j.cnki.syfjx.2012.16.017

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导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响

药理 | 更新时间：2024-09-23

- 导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响
- Inhibition of Daotan Decoction on the Expression of ICAM-1 and p21 in Carotid Atherosclerosis Rabbits
- 中国实验方剂学杂志 2012年18卷第16期页码：154-157
- 作者机构：
  
  1. 首都医科大学宣武医院
  2. 北京中医药大学附属东方医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30772843,30801481,81102624）;北京市科技新星项目（2008A90）;北京市中医药科技项目（JJ 2009-037）
- DOI：10.13422/j.cnki.syfjx.2012.16.017
  中图分类号： R285.5
- 纸质出版日期：2012
- 稿件说明：
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[1]陈文强,黄小波,李宗信,王芬,王宁群.导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响[J].中国实验方剂学杂志,2012,18(16):154-157.

CHEN Wen-qiang1, HUANG Xiao-bo1, LI Zong-xin1, et al. Inhibition of Daotan Decoction on the Expression of ICAM-1 and p21 in Carotid Atherosclerosis Rabbits[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 154-157.
[1]陈文强,黄小波,李宗信,王芬,王宁群.导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响[J].中国实验方剂学杂志,2012,18(16):154-157. DOI： 10.13422/j.cnki.syfjx.2012.16.017.

CHEN Wen-qiang1, HUANG Xiao-bo1, LI Zong-xin1, et al. Inhibition of Daotan Decoction on the Expression of ICAM-1 and p21 in Carotid Atherosclerosis Rabbits[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 154-157. DOI： 10.13422/j.cnki.syfjx.2012.16.017.

摘要

目的:研究导痰汤是否能通过对内皮细胞黏附分子-1（intercellular adhesion molecular-1

ICAM-1）和p21通路的调节而干预颈动脉粥样硬化。方法:将25只雄性新西兰白兔随机分为正常对照组、模型组、导痰汤治疗组（分为高、中、低剂量组）。对照组给予基础饲料喂饲

模型组和导痰汤治疗组予以高脂饲料喂饲

并在喂饲1周后实施颈动脉内膜空气干燥术

术后导痰汤治疗组分别按照10

6 g.kg-1

ig每日给药1次。4周后处死家兔

取每只家兔右侧颈动脉远端1 cm

固定后行HE染色

观察病理改变。应用免疫组化SABC法检测ICAM-1和p21表达情况。结果:模型组血管内皮细胞、平滑肌细胞的胞浆中有ICAM-1（0.200±0.035）和p21（0.297±0.097）强阳性表达

显著高于对照组ICAM-1（0.037±0.005）

p21（0.078±0.016）

（P<0.01）。导痰汤低、中、高剂量治疗组细胞内ICAM-1（0.166±0.043

0.104±0.011

0.098±0.010）和p21（中剂量:0.173±0.021

高剂量:0.128±0.026）阳性表达显著低于模型组（P<0.05）

且随剂量的增加

导痰汤对ICAM-1和p21的抑制逐渐增强;而p21与ICAM-1水平呈显著正相关（r=0.772

P<0.01）。结论:导痰汤可以有效抑制颈动脉硬化家兔血管内皮细胞中ICAM-1和p21的表达。

Abstract

Objective:To study the influence of Daotan decoction on the expression of intercellular adhesion molecular-1（ICAM-1） and p21 in carotid atherosclerosis rabbits.Method: Twenty-five male rabbits were divided into control group

model group and Daotan decoction group.Control group was treated with basic diet;model group and Daotan decoction group were treated with both high-fat diet and carotid artery intima air drying operation.Daotan decoction group was treated with Daotan decoction

at dose of 10

6 mg · kg-1

for 4 weeks.Then the right carotid was cut and fixed.The expression of ICAM-1 and p21 was assayed by immunohistochemistry and pathological staining.Result: Compared with control group（ICAM-1:0.037±0.005

p21:0.078±0.016）

the expression of ICAM-1（0.200±0.035） and p21（0.297±0.097） in model group was significantly increased（P<0.01）.And compared with model group

Daotan decoction could significantly restrain ICAM-1（low dose group: 0.166±0.043

medium dose group: 0.104±0.011

high dose group: 0.098±0.010） and p21（medium dose group: 0.173±0.021

high dose group: 0.128±0.026） expression（P<0.05）.Also

there was significant positive relationship between the expression of ICAM-1 and p21（r=0.772

P<0.01）.Conclusion: Daotan decoction could restrain ICAM-1 and p21 expression in carotid atherosclerosis rabbits.

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