Objective:To observe the effects of Jingqianshu granule on distribution and expression of estrogen receptor（ER） α and ERβ in the hypothalamus of premenstrual syndrome model rats with liver-qi stagnation

and to find out the corresponding effect targets and mechanisms of Jingqianshu granule.Method: Thirty female SD rats were randomly divided into three groups: the normal group

the model group

and the medication group.The premenstrual syndrome（PMS） model rats with Liver-qi stagnation were induced by bondage stimulation.Jingqianshu granule was given to rats in the medication group at the dose of 10 g · kg-1 by gastrogavage while equal volume of sterilized water was given to modeling.rats in the model group and the normal group

once daily.The protein distribution and integrated optical density（A） in ventromedial hypothalamic area（VMH） of rats in the three groups were detected by using immunohistochemistry ABC methods.HPLC method was used to detect 5-HT level in hypothalamus.Result: IHC demonstrated that there were no noticeable differences in the distribution of ERα

ERβ in VMH of hypothalamus in three groups.The IA of ERα

ERβ in model group which were 163 605±27 284 and 147 318±24 924 increased markedly compared with the normal group（130 156±32 579

105 430±28 641）（P<0.05

P<0.01）.The IA of ERα and ERβ of medication group which were 122 0120±21 287 and 77 482±31 112 decreased markedly compared with model group（all P<0.01）.The 5-HT level（454.51±65.18） μg · L-1 in hypothalamus of model group were noticeable increased compared with the normal group（144.47±42.20）μg · L-1（P<0.01）

and the 5-HT level（234.11±14.38）μg · L-1 of medication group decreased markedly compared with model group（P<0.01）.Conclusion: The high expressions of the ERα and ERβ protein in VMH may have close relationship with liver-qi stagnation

and probably is one of the centre pathogenesis of PMS with liver-qi stagnation.The Jingqianshu granule can correct the high expression of ERα and ERβ protein of model rats in VMH

which may be one of the mechanism and effect target for Jingqianshu granule.