ZHANG Qiang, DUAN Yu-min, WANG Wei-ming, et al. Morphologic Influence of Compound Qinglongyi Capsules on Apoptosis in Human Gastric Cancer Cell SGC-7901[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 181-183.
ZHANG Qiang, DUAN Yu-min, WANG Wei-ming, et al. Morphologic Influence of Compound Qinglongyi Capsules on Apoptosis in Human Gastric Cancer Cell SGC-7901[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 181-183. DOI: 10.13422/j.cnki.syfjx.2012.16.034.
透射电镜观察其对SGC-7901细胞超微结构影响;荧光显微镜下观察其对SGC-7901细胞凋亡形态的影响。结果:以不同浓度的复方青龙衣处理人胃癌细胞SGC-7901 48 h
透射电镜结果显示
对SGC-7901细胞的超微结构有影响
提示可诱导细胞凋亡;荧光显微镜结果显示
经Hoechest33258染液染色后的SGC-7901细胞随着给药剂量的增大
凋亡细胞比例逐渐增加。结论:复方青龙衣胶囊可诱导人胃癌细胞SGC-7901细胞发生凋亡。
Abstract
Objective:To investigate the morphologic influence of compound Qinglongyi capsules on apoptosis as well as to preliminarily study the basis for its growth inhibitory effect on human gastric cancer cell.Method: Preprocessing human gastric cancer cell SGC-7901 for 48 hours by setting 3 different drug concentration gradients 2.0×10-3
1.0×10-3
0.5×10-3 g·L-1 observing its effect on ultrastructure of SGC-7901 cell by transmission electron microscope;observing its effect on apoptosis of SGC-7901 cell by fluorescence microscope.Result: After dealing with human gastric cancer cell SGC-7901 with compound Qinglongyi capsules of different concentration for 48 hours
the result of transmission electron microscope showed that the drughad effect on the ultrastructure of SGC-7901 cell and indicated that it can induce apoptosis.The result of fluorescence microscope showed that with the increase of the dose
the apoptosis of SGC-7901 cells gradually increased in proportion after having been stained with Hoechest 33258 staining solution.Conclusion: Compound Qinglongyi capsule has obvious growth inhibitory effect on human gastric cancer cell SGC-7901
and it is probably able to induce apoptosis of cancer cells.