黄连解毒汤对神经细胞保护作用活性成分的筛选

彭扬中; 崔海峰; 冯淑怡; 孙明杰; 于友华; 孙丽华

doi:10.13422/j.cnki.syfjx.2012.16.040

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黄连解毒汤对神经细胞保护作用活性成分的筛选

药理 | 更新时间：2024-09-23

- 黄连解毒汤对神经细胞保护作用活性成分的筛选
- Screening of Active Constituents from Huanglian Jiedu Decoction for Protective Effect on Neurocytes
- 中国实验方剂学杂志 2012年18卷第16期页码：203-207
- 作者机构：
  
  中国中医科学院医学实验中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目（30873358）;中国中医科学院自主选题（ZZ2010011）
- DOI：10.13422/j.cnki.syfjx.2012.16.040
  中图分类号： R285.5
- 纸质出版日期：2012
- 稿件说明：
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[1]彭扬中,崔海峰,冯淑怡,孙明杰,于友华,孙丽华.黄连解毒汤对神经细胞保护作用活性成分的筛选[J].中国实验方剂学杂志,2012,18(16):203-207.

PENG Yang-zhong, CUI Hai-feng, FENG Shu-yi, et al. Screening of Active Constituents from Huanglian Jiedu Decoction for Protective Effect on Neurocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 203-207.
[1]彭扬中,崔海峰,冯淑怡,孙明杰,于友华,孙丽华.黄连解毒汤对神经细胞保护作用活性成分的筛选[J].中国实验方剂学杂志,2012,18(16):203-207. DOI： 10.13422/j.cnki.syfjx.2012.16.040.

PENG Yang-zhong, CUI Hai-feng, FENG Shu-yi, et al. Screening of Active Constituents from Huanglian Jiedu Decoction for Protective Effect on Neurocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 203-207. DOI： 10.13422/j.cnki.syfjx.2012.16.040.

摘要

目的:探讨黄连解毒汤及其主要成分小檗碱、黄芩苷、巴马汀、栀子苷对过氧化氢（H2O2）、谷氨酸（Glu）所致神经细胞损伤的影响。方法:制备H2O2

Glu损伤人神经母细胞瘤细胞（SH-SY5Y）、小鼠神经母细胞瘤细胞（Neuro-2a）模型

设正常对照、模型、黄连解毒汤、小檗碱、黄芩苷、巴马汀及栀子苷组

筛选浓度为7.812 5

15.625

31.25

62.5

125

250

500 mg.L-1

应用MTT法检测药物对损伤后细胞增殖的影响。结果:黄连解毒汤能够促进SH-SY5Y细胞增殖

适当浓度的栀子苷能够促进SH-SY5Y

Neuro-2a细胞增殖

并对H2O2

Glu损伤的SH-SY5Y细胞有保护作用

对H2O2损伤的Neuro-2a也具有保护作用。结论:栀子苷可能为黄连解毒汤对神经细胞保护作用的有效成分。

Abstract

Objective:To study the effect of Huangliang Jiedu decoction and its ingredients berberine

baicalin

palmatine

geniposide on the proliferation of neurocytes injured by H2O2 and glutamic acid（Glu）.Method: H2O2 and Glu were respectively used to induce the injured models of SH-SY5Y and Neuro-2a cell lines.The screening concentration of Huangliang Jiedu decoction

berberine

baicalin

palmatine

geniposide were 7.812 5

15.625

31.25

62.5

125

250

500 mg · L-1 respectively.MTT analysis was employed to evaluated the proliferation effect of cells after the injury.Result: Huangliang Jiedu decoction enhanced the proliferation of SH-SY5Y cell line.Geniposide in a reasonable concentration promoted the proliferation of SH-SY5Y and Neuro-2a cell lines.It also had the protective effects on SH-SY5Y cell line which were damaged by H2O2 and Glu.In addition

geniposide increased the proliferation of Neuro-2a cell line injured by H2O2.Conclusion: Geniposide might be the active ingredient of Huangliang Jiedu decoction to protect the injuried neurocyte.

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