薄荷总酚酸大孔吸附树脂纯化工艺优选

姜艳艳; 张凡; 刘斌

doi:10.13422/j.cnki.syfjx.2012.16.054

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薄荷总酚酸大孔吸附树脂纯化工艺优选

工艺与制剂 | 更新时间：2024-09-23

- 薄荷总酚酸大孔吸附树脂纯化工艺优选
- Optimization of Purification Technology for Total Phenolic Acid from Mentha haplocalyx by Macroporous Resin
- 中国实验方剂学杂志 2012年18卷第16期页码：9-11
- 作者机构：
  
  1. 北京中医药大学
  2. 国家中医药管理局中药经典名方有效物质发现重点研究室
- 作者简介：
- 基金信息：
  
  高等学校博士学科点专项科研基金（20100013110002）;国家自然科学基金项目（81173520）
- DOI：10.13422/j.cnki.syfjx.2012.16.054
  中图分类号： R284.1
- 纸质出版日期：2012
- 稿件说明：
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[1]姜艳艳,张凡,刘斌.薄荷总酚酸大孔吸附树脂纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):9-11.

JIANG Yan-yan1, ZHANG Fan1, LIU Bin1. Optimization of Purification Technology for Total Phenolic Acid from Mentha haplocalyx by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 9-11.
[1]姜艳艳,张凡,刘斌.薄荷总酚酸大孔吸附树脂纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):9-11. DOI： 10.13422/j.cnki.syfjx.2012.16.054.

JIANG Yan-yan1, ZHANG Fan1, LIU Bin1. Optimization of Purification Technology for Total Phenolic Acid from Mentha haplocalyx by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 9-11. DOI： 10.13422/j.cnki.syfjx.2012.16.054.

摘要

目的:优选大孔吸附树脂纯化薄荷总酚酸的工艺条件。方法:以总酚酸含量和吸附-解吸率为指标

考察5种不同大孔吸附树脂对总酚酸的吸附能力

确定最佳树脂型号

并优化其纯化工艺条件。结果:选用HPD-400型大孔吸附树脂

最佳工艺条件为上样液质量浓度0.1 g.mL-1

上样液pH 3

上样量6 BV

树脂柱径高比1∶9

2 BV 20%乙醇洗脱除杂

流速2BV.h-1

3 BV 70%乙醇以4 BV.h-1流速洗脱

收集洗脱液

薄荷总酚酸纯度可达53%。结论:所建立的工艺分离效果良好

操作简单

重复性好

可用于分离富集薄荷总酚酸。

Abstract

Objective:To optimize purification technology of total phenolic acid from Mentha haplocalyx by macroporous resin.Method: With the content of total phenolic acid and adsorption-resolution rate as indexes

optimum resin model was determined by investigating adsorption capacity of five different macroporous resin on total phenolic acid

and optimized its purification technology conditions.Result: HPD-400 macroporous resin was selected

Optimum purification conditions were as follow:The concentration of sample liquid was 0.1 g · mL-1 and then turn to pH 3 with acetic acid

the amount of sample 6 BV

ratio of diameter-height 1∶9

2 BV 20% ethanol to wipe off impurity with flow velocity of 2 BV · h-1

3 BV 70% ethanol to elute with 4 BV · h-1

eluate collected

purity of total phenolic acid 53%.Conclusion: Optimized purification technology was fit for separating and easy to operate with good reproducibility

it could be used to separate and enrichment total phenolic acid from M.haplocalyx.

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