大孔树脂对黄独总皂苷的分离纯化工艺优选

李春红; 梅志强; 田吉; 刘艳

doi:10.13422/j.cnki.syfjx.2012.16.060

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大孔树脂对黄独总皂苷的分离纯化工艺优选

工艺与制剂 | 更新时间：2024-09-23

- 大孔树脂对黄独总皂苷的分离纯化工艺优选
- Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin
- 中国实验方剂学杂志 2012年18卷第16期页码：27-29
- 作者机构：
  
  1. 泸州医学院药物与功能性食品研究中心
  2. 泸州医学院医学分子生物学教研室
- 作者简介：
- 基金信息：
  
  泸州医学院青年基金项目（2012026）
- DOI：10.13422/j.cnki.syfjx.2012.16.060
  中图分类号： R284
- 纸质出版日期：2012
- 稿件说明：
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[1]李春红,梅志强,田吉,刘艳.大孔树脂对黄独总皂苷的分离纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):27-29.

LI Chun-hong1, MEI Zhi-qiang2, TIAN Ji1, et al. Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 27-29.
[1]李春红,梅志强,田吉,刘艳.大孔树脂对黄独总皂苷的分离纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):27-29. DOI： 10.13422/j.cnki.syfjx.2012.16.060.

LI Chun-hong1, MEI Zhi-qiang2, TIAN Ji1, et al. Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 27-29. DOI： 10.13422/j.cnki.syfjx.2012.16.060.

摘要

目的:优选黄独总皂苷的分离纯化工艺。方法:以黄独总皂苷含量为指标

采用紫外分光光度法测定其含量

动态吸附-解吸法筛选大孔树脂型号

并优选黄独总皂苷的分离纯化工艺。结果:D-101型大孔树脂富集纯化黄独总皂苷的最佳工艺为黄独提取液质量浓度0.2 g.mL-1

上样量6 BV

流速1.0 mL.min-1

加4 BV水洗除杂

50%乙醇4 BV解吸

收集洗脱液。此工艺条件下所得提取物黄独总皂苷质量分数61.85%。结论:该优选工艺简单可行

能满足大生产实际要求。

Abstract

Objective:To optimize separation and purification technology of total saponins from Dioscorea bulbifera.Method: With the content of total saponins from D.bulbifera as index

which was determined by ultraviolet spectrophotometry

optimum macroporous resin model was selected by dynamic adsorption-desorption method

then separation and purification technology of total saponins from D.bulbifera was optimized.Result: Optimum separation and purification technology conditions of total saponins by D-101 macroporous resin were as follows:the concentration of extraction liquid from D.bulbifera 0.2 g · mL-1

sample volume of 6 BV

flow rate of 1.0 mL · min-1

washed impurity with 4 BV water

and then desorpted with 4 BV 30% ethano1

collected eluate.Under these technology conditions

yield of total saponins from D.bulbifera reached as high as 61.85%.Conclusion: This optimized technology was simple and feasible

which could meet industrial requirements.

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