大孔树脂精制纯化牡丹皮中芍药苷工艺考察

杨人泽; 丁乐; 魏桂林

doi:10.13422/j.cnki.syfjx.2012.16.065

您当前的位置：

首页 >

文章列表页 >

大孔树脂精制纯化牡丹皮中芍药苷工艺考察

工艺与制剂 | 更新时间：2024-09-23

- 大孔树脂精制纯化牡丹皮中芍药苷工艺考察
- Investigation of Separation and Purification Technology for Paeoniflorin from Paeonia suffruticosa by Macroporous Resin
- 中国实验方剂学杂志 2012年18卷第16期页码：42-44
- 作者机构：
  
  1. 赣南医学院第一附属医院
  2. 赣州市妇幼保健院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.065
  中图分类号： R284.1
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
[1]杨人泽,丁乐,魏桂林.大孔树脂精制纯化牡丹皮中芍药苷工艺考察[J].中国实验方剂学杂志,2012,18(16):42-44.

YANG Ren-ze1, DING Le2, WEI Gui-lin1. Investigation of Separation and Purification Technology for Paeoniflorin from Paeonia suffruticosa by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 42-44.
[1]杨人泽,丁乐,魏桂林.大孔树脂精制纯化牡丹皮中芍药苷工艺考察[J].中国实验方剂学杂志,2012,18(16):42-44. DOI： 10.13422/j.cnki.syfjx.2012.16.065.

YANG Ren-ze1, DING Le2, WEI Gui-lin1. Investigation of Separation and Purification Technology for Paeoniflorin from Paeonia suffruticosa by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 42-44. DOI： 10.13422/j.cnki.syfjx.2012.16.065.

摘要

目的:考察大孔树脂富集牡丹皮中芍药苷的工艺参数。方法:以芍药苷为指标

HPLC测定指标成分含量

采用静态、动态吸附-解吸试验考察大孔树脂型号并优选其富集纯化工艺条件。结果:采用AB-8型大孔树脂

上样液质量浓度0.125 g.mL-1

上样量2.25 BV

上样液pH 4

吸附流速2 BV.h-1

8 BV水洗除杂

14 BV 20%乙醇洗脱芍药苷

洗脱流速4BV.h-1。此条件下

芍药苷洗脱率达90.40%。结论:该优选工艺可较好的富集牡丹皮中有效成分芍药苷

可推广于工业化大生产。

Abstract

Objective:To investigate enrichment technology parameters of paeoniflorin from Paeonia suffruticosa by macroporous resin.Method: With paeoniflorin as index

which was determined by HPLC

model of macroporous resin was optimized by static and dynamic adsorption-desorption test

and to optimize its enrichment and purification technology conditions.Result: Optimal model of macroporous resin was AB-8 type

the mass concentration of sample liquid 0.125 g · mL-1

sample volume 2.25 BV

pH of sample liquid 4

adsorption flow rate 2 BV · h-1

washed impurity with 8 BV water

paeoniflorin was eluated with 14 BV 20% ethanol

desorption flow rate 4 BV · h-1.Under these conditions

elution rate of paeoniflorin was up to 90.40%.Conclusion: This optimized process could be well enrichment of paeoniflorin from P.suffruticosa

it could be promoted in industrial production.

关键词

Keywords

references

浏览量

149

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

连翘子总酚的树脂纯化工艺优选

强志怡神胶囊的大孔树脂纯化工艺优选

大孔树脂纯化豆豉溶栓酶的工艺优选

芍药苷干预Hippo信号通路诱导非小细胞肺癌凋亡的机制