GENG Fang1, WANG Fa-shan2, ZHU Kui-yuan3, et al. GC-MS Fingerprint of Wujia Shenghua Capsule[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 89-92.
GENG Fang1, WANG Fa-shan2, ZHU Kui-yuan3, et al. GC-MS Fingerprint of Wujia Shenghua Capsule[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 89-92. DOI: 10.13422/j.cnki.syfjx.2012.16.077.
Objective:To establish GC-MS fingerprint of Wujia Shenghua capsule.Method: Chromatographic conditions: HP-5MS quartz capillary column(0.25 μm×0.25 mm×30 m);temperature program carrier gas(He)
velocity 1.0 mL · min-1
the injection volume 1 μL;split ratio 1 ∶ 20.MS conditions: electron impact(EI) ion source
transmission line temperature 280 ℃
ion source temperature 230 ℃
quadrupole temperature 150 ℃ mass scan range m/z 30-600.And the results were contrasted with Chemstation and Nist 05a.Result: Seven peaks were found in the GC-MS specific chromatogram of volatile oil from Wujia Shenghua capsule
and six main common peaks were appraised.The operating standard of Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004 A) was used to calculate.Similarity of 11 batches of Wujia Shenghua capsule decoction samples was higher than 0.90.Conclusion: The fingerprinting could be used as a method of quality control and provide evidence for the research of effective components of Wujia Shenghua capsule.