Objective:To develop a HPLC method for determining 6 amino acids in Lujiao Buxue keli.Method: The sample was derived with phenyl isothiocyanate（PITC）.Amino acids were separated on Hypersil BDS C18（4.6 mm×200 mm

5 μm） column at the flow rate of 1.0 mL · min-1

detected at 254 nm.Result: L-Glu had good linearity in the range of 0.169-1.014 μg（r=0.999 5）.The average recovery was 101.43%

RSD was 3.20%;L-Hyp had good linearity in the range of 0.178-1.068 μg（r=0.999 9）.The average recovery was 101.23%

RSD was 3.25%;Gly had good linearity in the range of 0.381-2.286 μg（r=0.999 5）.The average recovery was 102.87%

RSD was 2.32%;L-Ala had good linearity in the range of 0.162-0.972 μg（r=0.999 5）.The average recovery was 102.37%

RSD was 3.36%;L-Arg had good linearity in the ranges of 0.118-0.708 μg（r=0.999 6）.The average recovery was 102.73%

RSD was 2.41%;L-Pro had good linearity in the range of 0.253-1.518 μg（r=0.999 9）.The average recovery was 98.69%

RSD was 2.34%. Conclusion: The method is reliable

which is helpful for the determination of amino acids in Lujiao Buxue keli.