Objective:To establish specific chromatogram of compound Jinyinhua granule by using HPLC for quality evalution and to determin the conten of chlorogenic acid

baicaliin and forsythin in compound Jinyinhua granule.Method: The analysis was performed on a Phenomenex C18 column（4.6 mm×250 mm

4 μm）with acetonitrile-0.4% phosphoric acid as mobile phase in a gradient mode at a flow rate of 1.0 mL · min-1

and at a column temperature of 35 ℃.The detection wavelength was at 228 nm.Recorded time was 80 min.Result: The specific chromatogram of compound Jinyinhua granule was established and 13 common peaks were indicated.Through reference substances

3 common peak were identified.The relative standard deviations for re1ative retention times and relative peak areas were less than 4% in the precision and repeated test.Base line resolution was achieved.The linear ranges of chlorogenic acid

baicaliin and forsythin were 0.11-5.57 μg（r=0.999 9）

0.05-2.66 μg（r=1）

0.01-0.52 μg（r=1）

respectively.The average recoveries were 98.9%

100.3%

99.2%

respective1y.Conclusion: The method is accurate

feasible and reliable

which can be used for the quality of compound Jinyinhua granule.