抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选

刘晓亚; 李志慧; 王巍; 刘倩; 谢飞; 曹艳玲; 韦灵玉; 张园园; 包巨太; 张玉杰

doi:10.13422/j.cnki.syfjx.2012.18.019

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抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选

制剂与工艺 | 更新时间：2024-09-23

- 抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选
- Optimization of Purification Technology for Alcohol Extract of K-CoxB-JN by Macroporous Resin
- 中国实验方剂学杂志 2012年18卷第18期页码：4-6
- 作者机构：
  
  1. 北京中医药大学中药学院
  2. 河北联合大学中医学院
  3. 哈尔滨商业大学中药学院
- 作者简介：
- 基金信息：
  
  国家重大新药创制科技重大专项课题（2009ZX09103-442）
- DOI：10.13422/j.cnki.syfjx.2012.18.019
  中图分类号： R286
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
[1]刘晓亚,李志慧,王巍,刘倩,谢飞,曹艳玲,韦灵玉,张园园,包巨太,张玉杰.抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选[J].中国实验方剂学杂志,2012,18(18):4-6.

LIU Xiao-ya1, LI Zhi-hui1, WANG Wei2, et al. Optimization of Purification Technology for Alcohol Extract of K-CoxB-JN by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 4-6.
[1]刘晓亚,李志慧,王巍,刘倩,谢飞,曹艳玲,韦灵玉,张园园,包巨太,张玉杰.抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选[J].中国实验方剂学杂志,2012,18(18):4-6. DOI： 10.13422/j.cnki.syfjx.2012.18.019.

LIU Xiao-ya1, LI Zhi-hui1, WANG Wei2, et al. Optimization of Purification Technology for Alcohol Extract of K-CoxB-JN by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 4-6. DOI： 10.13422/j.cnki.syfjx.2012.18.019.

摘要

目的:优选抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺。方法:以总皂苷、总黄酮和总木脂素含量为指标

考察树脂的吸附和解吸能力

筛选大孔树脂型号

采用单因素试验对最大上样量、上样流速、洗脱溶剂等进行考察。结果:非极性D101型大孔树脂效果最佳

优选的纯化工艺条件为醇提液生药质量浓度0.5 g.mL-1

药材-湿树脂量1∶4

吸附流速2 BV.h-1

3 BV水洗除杂

4 BV 70%乙醇洗脱。在此工艺条件下

总皂苷、总黄酮和总木脂素纯度分别由5.36%

0.3%

2.26%提高到53.82%

3.01%

22.87%。结论:D101型大孔树脂纯化抗柯萨奇B病毒性心肌炎胶囊醇提物

方法简便可行

纯化效果好

可推广使用。

Abstract

Objective:To optimize purification technology of total saponins

flavonoids and lignins from K-CoxB-JN by macroporous resin.Method:Taking the contents of total saponins

flavonoids and lignins as indexes

adsorption and desorption properties of 10 kinds macroporous resins were compared

based on these

maximum sample volume

sample flow rate and elution solvent were investigated by single-factor test.Result:D101 macroporous resin gave good static-dynamic and adsorption-desorption characteristics for all components

optimized purification technology was:crude drug concentration of alcohol extraction solution 0.5 g·mL-1

herbs-wet resin 1 ∶ 4

adsorption flow rate 2 BV·h-1

washed with 3 BV distilled water

4 BV of 70% ethanol as elution solvent.Under these conditions

purity of total saponins

total flavonoids and total lignins were enriched from 5.36%

0.3%

2.26% to 53.82%

3.01%

22.87%.Conclusion:This optimum purification technology of K-CoxB-JN was simple

stable and effective with good purification effect

it could be promoted in large-scale production.

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