赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析

徐秀泉; 虞倩; 徐颖; 许慧敏

doi:10.13422/j.cnki.syfjx.2012.18.029

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赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析

制剂与工艺 | 更新时间：2024-09-23

- 赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析
- Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Penthorum chinense by Response Surface Methodology and Evaluation Its in vitro Antioxidant Activity
- 中国实验方剂学杂志 2012年18卷第18期页码：38-41
- 作者机构：
  
  江苏大学药学院
- 作者简介：
- 基金信息：
  
  江苏大学大学生科研项目（11A155）
- DOI：10.13422/j.cnki.syfjx.2012.18.029
  中图分类号： R284.1
- 纸质出版日期：2012
- 稿件说明：
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[1]徐秀泉,虞倩,徐颖,许慧敏.赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析[J].中国实验方剂学杂志,2012,18(18):38-41.

XU Xiu-quan, YU Qian, XU Ying, et al. Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Penthorum chinense by Response Surface Methodology and Evaluation Its in vitro Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 38-41.
[1]徐秀泉,虞倩,徐颖,许慧敏.赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析[J].中国实验方剂学杂志,2012,18(18):38-41. DOI： 10.13422/j.cnki.syfjx.2012.18.029.

XU Xiu-quan, YU Qian, XU Ying, et al. Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Penthorum chinense by Response Surface Methodology and Evaluation Its in vitro Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 38-41. DOI： 10.13422/j.cnki.syfjx.2012.18.029.

摘要

目的:优化赶黄草总黄酮的超声提取工艺并分析其体外抗氧化活性。方法:以总黄酮得率为指标

在单因素试验基础上

采用响应面设计法对影响总黄酮得率的乙醇体积分数、超声时间及料液比进行优化

同时考察其体外抗氧化活性。结果:赶黄草总黄酮超声提取的最佳工艺条件为26倍量60%乙醇于50℃下超声提取20 min。在此条件下

总黄酮得率4.14%

试验结果与模型预测值相符。体外抗氧化活性研究表明

赶黄草总黄酮具有较强的还原力和总抗氧化能力

同时具有较强的清除羟基自由基和DPPH自由基能力。结论:该优选工艺方便、快捷、高效;赶黄草总黄酮具有较强的抗氧化活性

值得深入研究。

Abstract

Objective:To optimize ultrasonic-assisted extraction technology of total flavonoids from Penthorum chinense and evaluate its in vitro antioxidant activity.Method:With yield of total flavonoids as index

the concentration of ethanol

extraction time

liquid-material ratio were optimized by response surface methodology based on single-factor tests

and its in vitro antioxidant activity were evaluated by multi-test systems.Result:Optimal extraction technology conditions were:extracted 20 min with 26 times the amount of 60% ethanol at 50 ℃.Under these conditions

yield of total flavonoids reached 4.14%

which was agreed with model predictions.In vitro antioxidant activities results showed that total flavonoids from P.chinense had good antioxidant activities of scavenging DPPH free radical and hydroxyl free radical

but also had strong reducing power and total antioxidant capability.Conclusion:Optimized ultrasonic-assisted extraction technology was simple

rapid and efficient.Total flavonoids from P.chinense had good antioxidant activity which was worth of further study.

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