Objective:To analyze transdermal effect and transdermal composition of volatile oil from Notopterygium incisum

Angelica pubescens and pair of them by GC-MS.Method:Chemical constituents of volatile oil in in vitro transdermal donor cell and receiving cell were identified by GC-MS

The relative contents in percentage were calculated with area normalization method.According to mathematical derivation

relative contents was changed into translation rate

which reflected transdermal effect of every components.Result:After percutaneous absorption

thirty-nine components were identified in N.incisum（93.22%）

twenty-nine in A.pubescens（93.74%） and thirty-six in drug pair of them（92.75%）.Main components of percutaneous absorption in N.incisum were 1-terpineol（30.96%）

4-terpineol（28.22%）

borneol（7.10%）

etc;Main components of percutaneous absorption in A.pubescens were o-acetyl-p-methylbenzyl alcohol（55.70%）

trans-oxy-linalool（6.79%）

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7

7-trimethyl-bicyclic 5-hexene-2-one（4.78%）

etc;Main components of percutaneous absorption in drug pair of them were 1-terpineol（28.86%）

4-terpineol（22.41%）

o-acetyl-p-methylbenzyl alcohol（12.15%）

borneol（5.62%）

etc.Conclusion:Transmission rate of every components in three kinds of volatile oil could be sorted qualitatively of transdermal effect.Transmission rate of 1-terpineol was the most in volatile oil of N.incisum.1

7

7-trimethyl-bicyclic 5-hexene-2-one had the most transmission rate in volatile oil of A.pubescens.1-terpineol had the most transmission ratio in volatile oil of drug pair of them.