SUN Wen, CHAO Zhi-mao, WANG Chun, et al. Determination of 5-Hydroxmethylfurfural in Commercial Trichosanthis Fructus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 73-76.
SUN Wen, CHAO Zhi-mao, WANG Chun, et al. Determination of 5-Hydroxmethylfurfural in Commercial Trichosanthis Fructus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 73-76. DOI: 10.13422/j.cnki.syfjx.2012.18.037.
Objective:To establish an HPLC method for content determination of 5-hydroxymethylfurfural(5-HMF) in commercial Trichosanthis Fructus
a commonly used Chinese material medica.Method:The HPLC analysis was performed on an ODS column(4.6 mm×250 mm
5 μm) and the mobile phase consisted of methanol-water(15∶85) at a flow rate of 0.8 mL · min-1.The detective wavelength was set at 284 nm and the column temperature was maiutain at 30 ℃.Result:The HPLC method for content determination of 5-HMF in Trichosanthis Fructus was established.5-HMF was detected in 18 batches of commercial Trichosanthis Fructus
the content of which were in the range of 11.10-193.6 μg·g-1.Conclusion:The content of 5-HMF in commercial Trichosanthis Fructus is quite different.This method is suitable for the quality control of Trichosanthis Fructus.