ZHANG Ming1, CHEN Hua-guo1, ZHAO Chao1, et al. Determination of Content of Total Flavonoids from Polygonum perfoliatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80.
ZHANG Ming1, CHEN Hua-guo1, ZHAO Chao1, et al. Determination of Content of Total Flavonoids from Polygonum perfoliatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80. DOI: 10.13422/j.cnki.syfjx.2012.18.038.
Objective:To establish a method for determination of total flavonoids in Polygonum perfoliatum.Method:Total flavoaoids were determined by UV-Vis spectrophotometer with rutiu as reference substance and 0.1 mol · L-1 AlCl3
1 mol · L-1 NaAc as chromogenic agent;the detection wavelength was at 413 nm.Result:A good linear relationship was achieved over the concentration range of 6.24-21.84 μg(r =0.999 8) for Rutin.The regression equation of total flavonoids was Y=38.183X-0.001
with an average recovery of 97.4%
RSD 2.2%.Conclusion:The method is simple and stable
and it is suitable for the content determination of total flavonoids from P.perfoliatum.