Objective:To study in vitro bacteriostasis of ethanol extract from the rhizomes of Zingiber striolatum Diels.Method:The rhizomes were percolated with ethanol and then the residue was sequentially extracted with petroleum ether

chloroform

ethyl acetate and n-butanol after reduce-pressure distillation.The ethanol extract and various polar fractions were screened for in vitro antibacterial activities by disc-diffusion method.The minimum inhibition concentrations（MICs） and minimum bacteriocidal concentrations（MBCs） were further determined for active polar fractions by broth microdilution method.Result:The crude ethanol extract（CEE）

petroleum ether-and chloroform soluble-parts（PE and CE

resp.） inhibited growth of Gram-positive（Staphylococcus aureus

Baicllus licheniformis and Streptococcus agalactiae） and Gram-negative（Escherichia coli

Klebsiella pneumonia and Pseudomonas aeruginosa） with MICs ranging from 312.5 mg · L-1 to 1 250 mg · L-1.The MBCs of CEE against B.licheniformis

S.agalactiae and E.coli were 1 250 mg · L-1

while those of PE and CE against all the test strains varied from 625 mg · L-1 to 1 250 mg · L-1.Conclusion:The CEE

PE and CE from the rhizomes of Z.striolatum possess antibacterial and bacteriocidal activities against S.aureus

B.licheniformis

S.agalactiae

E.coli

K.pneumonia and P.aeruginosa.