大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选

胡正芳; 柳燕; 贾树田; 李琴

doi:10.13422/j.cnki.syfjx.2013.03.025

您当前的位置：

首页 >

文章列表页 >

大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选

工艺与制剂 | 更新时间：2024-09-23

- 大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选
- Optimization of Purification Technology of Total Lignans from Schisandra sphenanthera in Conjunction with Macroporous Resin and Aluminum Oxide Column
- 中国实验方剂学杂志 2013年19卷第3期页码：36-39
- 作者机构：
  
  南京海陵中药制药工艺技术研究有限公司
- 作者简介：
- 基金信息：
  
  南京市科技发展计划项目（201002018）
- DOI：10.13422/j.cnki.syfjx.2013.03.025
  中图分类号： R284
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
[1]胡正芳,柳燕,贾树田,李琴.大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选[J].中国实验方剂学杂志,2013,19(03):36-39.

HU Zheng-fang, LIU Yan, JIA Shu-tian, et al. Optimization of Purification Technology of Total Lignans from Schisandra sphenanthera in Conjunction with Macroporous Resin and Aluminum Oxide Column[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 36-39.
[1]胡正芳,柳燕,贾树田,李琴.大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选[J].中国实验方剂学杂志,2013,19(03):36-39. DOI： 10.13422/j.cnki.syfjx.2013.03.025.

HU Zheng-fang, LIU Yan, JIA Shu-tian, et al. Optimization of Purification Technology of Total Lignans from Schisandra sphenanthera in Conjunction with Macroporous Resin and Aluminum Oxide Column[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 36-39. DOI： 10.13422/j.cnki.syfjx.2013.03.025.

摘要

目的:优选大孔树脂与氧化铝联用对南五味子总木脂素提取液的纯化工艺。方法:以除杂率和五味子酯甲转移率的综合评分为指标

采用正交试验考察树脂用量、乙醇体积分数和乙醇用量对南五味子总木脂素大孔树脂纯化工艺的影响;通过单因素试验考察南五味子总木脂素的氧化铝吸附除杂工艺。采用紫外分光光度法测定总木脂素含量

HPLC测定五味子酯甲、五味子甲素的含量。结果:最佳纯化工艺为取2/3～4/3倍量LSA-7型大孔树脂

加85%乙醇6 BV洗脱

洗脱液过1倍药材量氧化铝柱吸附除杂。结论:该优选工艺合理、稳定可行

适用于工业生产。

Abstract

Objective:To optimize purification technology of total lagnans from Schisandra sphenanthera extract by macroporous resin combined with aluminum oxide.Method:With composite score of impurity removal rate and transfer rate of schisantherin A as index

effect of the amount of resin

ethanol concentration and amount on macroporous resin purification technology of total lignans was investigated by L9（34） orthogonal test;alumina adsorption purifying technology of total lignans from S.sphenanthera was investigated by single factor test.The content of total lignans was determined UV spectrophotometry

HPLC was used to determine the contents of schisantherin A and deoxyschizandrin.Result:Optimum purification conditions of total ligans in S.sphenanthera were as follows:took 2/3-4/3 times the amount of LSA-7 macroporous resin

eluted with 6 BV 85% ethanol

ratio of aluminum oxide and crude drug 1∶ 1.Conclusion:This optimized purification process was feasible

stable

reasonable and suitable for industrial production.

关键词

Keywords

references

浏览量

302

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

大孔树脂与氧化铝联用纯化南五味子总木脂素的工艺优选

栀子总环烯醚萜和总西红花苷的提取纯化工艺考察

阴离子交换树脂对人参皂苷的吸附/洗脱特性考察及LC-MS分析

地榆成分在D101型大孔树脂和聚酰胺树脂上保留行为的LCMS-IT-TOF分析

小檗皮总生物碱提取物的大孔树脂纯化工艺与质量标准考察