多指标优化吴茱萸的提取纯化工艺

王群; 刘文; 宋信莉; 施晓伟; 陈大业; 杨颂

doi:10.13422/j.cnki.syfjx.2013.03.027

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多指标优化吴茱萸的提取纯化工艺

工艺与制剂 | 更新时间：2024-09-23

- 多指标优化吴茱萸的提取纯化工艺
- Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes
- 中国实验方剂学杂志 2013年19卷第3期页码：45-48
- 作者机构：
  
  贵阳中医学院
- 作者简介：
- 基金信息：
  
  贵州省中药现代化科技产业研究开发专项（黔科合中药[2010]5016）
- DOI：10.13422/j.cnki.syfjx.2013.03.027
  中图分类号： R284
- 纸质出版日期：2013
- 稿件说明：
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[1]王群,刘文,宋信莉,施晓伟,陈大业,杨颂.多指标优化吴茱萸的提取纯化工艺[J].中国实验方剂学杂志,2013,19(03):45-48.

WANG Qun, LIU Wen, SONG Xin-li, et al. Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 45-48.
[1]王群,刘文,宋信莉,施晓伟,陈大业,杨颂.多指标优化吴茱萸的提取纯化工艺[J].中国实验方剂学杂志,2013,19(03):45-48. DOI： 10.13422/j.cnki.syfjx.2013.03.027.

WANG Qun, LIU Wen, SONG Xin-li, et al. Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 45-48. DOI： 10.13422/j.cnki.syfjx.2013.03.027.

摘要

目的:优选吴茱萸的提取纯化工艺。方法:以吴茱萸内酯、吴茱萸碱、吴茱萸次碱转移率的综合评分为指标

采用正交试验考察溶媒倍数、溶剂浓度、提取次数和提取时间对吴茱萸醇提工艺的影响;以吴茱萸内酯、吴茱萸碱、吴茱萸次碱的质量分数为指标

通过单因素试验考察吴茱萸提取液逐步水沉纯化工艺。结果:最佳提取工艺为加16倍量70%乙醇回流提取3次

每次3 h;最佳逐步水沉纯化工艺为回收乙醇

离心

取沉淀加20倍量90%乙醇超声提取15 min

离心

取上清液

加1/4倍量水进行二次水沉。结论:优选的提取、纯化工艺可确保吴茱萸中有效成分最大限度保留。

Abstract

Objective:To optimize extraction and purification technology of Evodia rutaecarpa.Method:With tranfer rate of evodine

evodiamine and rutacarpine as comprehensive evaluation index

influence of extraction times

solvent multiples

solvent concentration and extraction time on alcohol extraction technology of E.rutaecarpa was investigated by orthogonal test;With the mass fraction of evodine

evodiamine and rutacarpine as indexes

single factor test was used to investigate progressive water precipitation purification technology of extraction solution.Result:Optimum extraction technology was as following:refluxing extracted 3 times with 16 times the amount of 70% ethanol

3 hours each time;optimum progressive water precipitation purification technology:recovery of ethanol

centrifugation

precipitate was ultrasonic extracted 15 min with 20 times the amount of 90% ethanol

centrifugation

taking the supernatant

added quarter times of water in second time of water precipitation.Conclusion:Optimized extraction and purification technology could ensure effective components from E.rutaecarpa to maximize retention.

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