Objective:To establish a method for determination of trachelogenin-4′-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin in Trachelospermum jasminoides.Method: The Alltima C18 column（4.6 mm×250 mm

5 μm） was used;the mobile phase consisting of 0.5%HAc-water.The flow rate was 1.0 mL · min-1;the detection wavelength set at 270 nm and content calculated with external reference method. Result: The five compounds were well separated.Calibration curves showed good linear relationship

RSD<2.0%.The average recoveries of trachelogenin-4′-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin were 98.27%

97.30%

97.20%

96.89% and 96.48% respectively.Conclusion: The developed method is simple

accurate

and repeatable

which can be readily used as valid tool for the quality control of T.Jasminoides.