QIN Lan-fang1, HU Qi-ming1, LAI Mao-xiang1, et al. Simultaneous Determination of Quercetin and Kaempferol in Leaf of Ligustrum sinense by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 107-109.
QIN Lan-fang1, HU Qi-ming1, LAI Mao-xiang1, et al. Simultaneous Determination of Quercetin and Kaempferol in Leaf of Ligustrum sinense by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 107-109. DOI: 10.13422/j.cnki.syfjx.2013.03.043.
Objective:To establish an analytical method on simultaneous determination of quercetin and kaempferol in leaf of Ligustrum sinense.Method: The analysis was carried out on a column eluted with a mobile phase of methanol-0.4% phosphoric acid solution(55∶45).The flow rate was 1.0 mL · min-1;the detection wavelength was set at 360 nm.Result: The calibration curves of quercetin and kaempferol were linear in the ranges of 0.058-0.525 μg and 0.203-0.712 μg
and the average recoveries were 103.0% and 107.6%(n=6)
respectively.Conclusion: The method is accurate
reliable and repeatable with stable result.It can be used as a method of quantitative determination of quercetin and kaempferol in the leaf of L.sinense