YAN Chao-li. HPLC-ELSD Determination of Peimine in Shelian Chuanbei Capsules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 116-118.
YAN Chao-li. HPLC-ELSD Determination of Peimine in Shelian Chuanbei Capsules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 116-118. DOI: 10.13422/j.cnki.syfjx.2013.03.046.
Objective:To establish the method of determining peimine in Shelian Chuanbei capsules.Method: HPLC-ELSD was used to determine peimine in Shelian Chuanbei capsules.The chromatographic analysis were carried on C18 column
The mobile phase composition was acetonitrile-water-diethylamine(70∶30∶0.3) at 35 ℃;Drift tube temperature was 70 ℃
gas flow rate was 2.0 L · min-1.Result: Peimine were separated well.The calibration curves was linear in the ranges of 0.18-1.08 μg.and regressive equations was Y=1.170 6X+6.26.Average recoveries was 99.79% Conclusion: The method is simple and rapid and with satisfactory results
It is suitable for quality control of Shelian Chuanbei capsules.