Objective:Relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection was studied by observing influnce of Mailuoning injection on release media of RBL-2H3 cell（rat basophilic leukemia cell line）.Method: Mailuoning containing chlorogenic acid was as a suject

and puerarin injection not containing as a negative control

while chlorogenic acid standard as a positive control

the influntion of RBL-2H3 cells model in vitro

the degranulation response and changes in cell morphology was observed

β-hexosaminidase release was detected.Result: The Mailuoning and chlorogenic acid can be a significant factor resulting in release of β-hexosaminidase from RBL-2H3 cells（n=5

P<0.05）

puerarin injection group could promote the release of β-hexosaminidase.Conclusion: The Mailuoning group abversely caused the RBL-2H3 cells releasing β-hexosaminidase

and there is some relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection.