芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β<sub>1</sub> mRNA蛋白表达的影响

陈桂敏; 梁振钰; 高湲

doi:10.13422/j.cnki.syfjx.2013.03.080

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芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β₁ mRNA蛋白表达的影响

药理 | 更新时间：2024-09-23

- 芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β₁ mRNA蛋白表达的影响
- Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF-β₁ mRNA Expression
- 中国实验方剂学杂志 2013年19卷第3期页码：249-251
- 作者机构：
  
  海南医学院中医学院
- 作者简介：
- 基金信息：
  
  海南省自然科学基金项目（808165）
- DOI：10.13422/j.cnki.syfjx.2013.03.080
  中图分类号： R285.5
- 纸质出版日期：2013
- 稿件说明：
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[1]陈桂敏,梁振钰,高湲.芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β_1 mRNA蛋白表达的影响[J].中国实验方剂学杂志,2013,19(03):249-251.

CHEN Gui-min, LIANG Zhen-yu, GAO Yuan. Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF-β₁ mRNA Expression[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 249-251.
[1]陈桂敏,梁振钰,高湲.芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β_1 mRNA蛋白表达的影响[J].中国实验方剂学杂志,2013,19(03):249-251. DOI： 10.13422/j.cnki.syfjx.2013.03.080.

CHEN Gui-min, LIANG Zhen-yu, GAO Yuan. Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF-β₁ mRNA Expression[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 249-251. DOI： 10.13422/j.cnki.syfjx.2013.03.080.

摘要

目的:探讨芪蚣抗纤方（QKD）及拆方抗免疫损伤性大鼠肝纤维化作用机制及配伍意义。方法:采用改良型血清药理学对比方法

观察各组血清对肝星状细胞（HSC-T6）增殖及转化生长因子（TGF-β1）mRNA受体蛋白表达的影响。结果:与正常大鼠血清组比较

模型组大鼠血清组HSC-T6增殖明显增强（P<0.01）;与模型组比较

药物血清各组能抑制HSC-T6增殖（P<0.01或P<0.05）。与正常对照组比较

模型组大鼠HSC-T6细胞上清液中TGF-β1mRNA含量明显升高（P<0.01）。药物血清各组TGF-β1mRNA含量均明显下调（P<0.01）。结论:芪蚣抗纤方及拆方抗肝纤维化的主要机制之一是抑制HSC-T6增殖

使TGF-β1mRNA受体蛋白表达下调

芪蚣抗纤方全方较活血方和软坚方更具配伍意义

且呈剂量依赖性。

Abstract

Objective:To analyze the mechanism of antifibrotic role of Qigong Kangxian decoction（QKD） and the component herbs in rat models of hepatic fibrosis induced by immune injury.Method: A modified comparison method of serum pharmacology was applied to analyze

and QKD’s impact on growth of hepatic stellate cell（HSC-T6） cell and expression of transforming growth factor（TGF-β1） receptor mRNA protein.Result: significant increase in cell growth of stellate cell（HSC-T6） were observed in the rat models comparing with that of normal healthy rats.While rat models treated with different QKD doses showed significant inhibitory effects on growth of HSC-T6 cell（P<0.01 or P<0.05） comparing with model rats.Comparing with normal control group

TGF-β1 mRNA concentration increased significantly in rat models（P<0.01）

but in rat models treated with QKD

TGF-β1 mRNA concentration were all regulated to decrease obviously（P<0.01）.Conclusion: One of the main mechanisms of antifibrotic role of QKD and individual herbs is the inhibitory impact on HSC cell growth

and down-regulation of TGF-β1mRNA receptor protein expression.QKD shows a dose dependent effect

which is better than that of Huoxue formula and Ruanjian formula.

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芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β1 mRNA蛋白表达的影响

Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF-β1 mRNA Expression

DOI：10.13422/j.cnki.syfjx.2013.03.080

摘要

Abstract

关键词

Keywords

references

芪蚣抗纤方及拆方药物血清对HSC-T6增殖及TGF-β₁ mRNA蛋白表达的影响

Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF-β₁ mRNA Expression