LIN Sheng-de1, JIANG Dao-feng2, ZHANG Yi1, et al. Determination of Total Saponins from Different Medicinal Parts in Tibetan Medicine Pterocephalus hookeri by Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 101-104.
LIN Sheng-de1, JIANG Dao-feng2, ZHANG Yi1, et al. Determination of Total Saponins from Different Medicinal Parts in Tibetan Medicine Pterocephalus hookeri by Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 101-104. DOI: 10.13422/j.cnki.syfjx.2013.05.011.
Objective:To determine the total saponins from P.hookeri in different medicinal part and compare the differences in the different parts.Method: Ursolic acid was selected as control article.Coloration was used with 5% vanillin and perchloric acid.The content of total saponins from P.hookeri in different medicinal parts was determined at 550 nm by UV.Result: Ursolic acid was linear within the range of 0.058 2-0.203 8 mg;the correlation coefficient r=0.999 1.The average recovery was 97.06%
RSD 93%.Conclusion: The average content of total saponins from aerial parts was up to 5%.Combining Tibetan hospital drug use and pharmacological research status
in the premise of resource protection
and do not affect its curative effect at the same time
it is recommended that the aerial part of P.hookeri should be its medicinal part.