JIA Zhi-ruo1, LI Bing1, LI Yao-hua1, et al. Determination of Catechin in Eucommia ulmoids by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119.
JIA Zhi-ruo1, LI Bing1, LI Yao-hua1, et al. Determination of Catechin in Eucommia ulmoids by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119. DOI: 10.13422/j.cnki.syfjx.2013.05.057.
Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides.Method: The orthogonal test was used to optimize the pretreatment process.RP-HPLC was adopted
on a column of Agilent HC-C18(4.6 mm×250 mm
5 μm)
using the mobile phase of acetonitrile-0.4% phosphoric acid solution(13∶87)
at a flow rate of 1.0 mL·min-1;the detection wavelength was set at 280 nm.Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3).The average recovery of catechin was 97.38% with RSD 2.1%.Conclusion: The method is simple
rapid and reliable with good reproduciblity
which can be used for the content determination of catechin in E.ulmoides.