Objective:To investigate the effects of Xiaochaihu decoction（XCHD） containing drug serum on the cell proliferation of hepatocarcinoma HepG-2 cell.Method: XCHD containing drug serum was prepared.The cells were separated into four groups: 10% control serum group

5% XCHD containing drug serum

10% XCHD containing drug serum and 20% XCHD containing drug serum.The HepG-2 cell morphologic changes were observed using the inverted microscope;HepG-2 cell viability was measured by MTT assay.Apoptosis was detected by flow cytometry assay.Mitochondrial membrane potential changes were measured using fluorescent rhodamine123.Result: Compared with the control group

XCHD containing drug serum could significantly inhibit the proliferation of HepG-2 cell and the inhibition effect of 10% XCHD containing drug serum exhibited time-dependent relationship;flow cytometry results showed that after being treated with 10% XCHD containing drug serum

the apoptosis rate of HepG-2 cell elevated from 6.78% to 19.51%;rhodamine123 staining revealed that the mitochondrial membrane potential was reduced.Conclusion: XCHD containing drug serum could inhibit the proliferation of hepatocarcinoma HepG-2 cells and promote the apoptosis rate.The main mechanism of XCHD inhibiting HepG-2 cell is related with mitochondrial function.