Objective:To study the protective effect of rhubarb on intestinal barrier injury of dogs with severe acute pancreatitis.Method: Fifteen healthy Beagel dogs were used to establish severe acute pancreatitis model by retrograde pancreatic duct injection and were randomly divided into sham group

model group and treatment group.The treatment group was given rhubarb anthraquinones of total extracted by stomach injection at 6 hours after modeling.Result: The contents of endotoxin in peripheral blood was less than sham operation group

at 7

11 hour after modeling was significantly lower（P<0.05）

after administration the level of endotoxin had the tendency of increase and 3 hours after drug administration it was significantly higher than the model group（P<0.05）.In early times of severe acute pancreatitis

peripheral blood amylase and tumor necrosis factor（TNF-α） was significantly increased

while the level of endotoxin was lower.Rhubarb anthraquinone had not significant affect on the serum amylase of SAP

it could inhibit the sustained increaseof peripheral blood TNF-α levels and increase in peripheral blood plasma levels of endotoxin in early time of SAP.Conclusion: The effect of Rhubarb on severe acute pancreatitis with endotoxin translocation in early time may be ralated to that it can inhibit the inflammatory response improve intestinal microcirculation and restore normal intestinal absorption.