Objective:To establish the HepG2 cells model of insulin resistance by the method of insulin-induced in vitro

and provide a reliable model of insulin resistance in vitro for the study of the insulin resistance and the type 2 diabetes.Method: RPMI 1640 medium with different insulin concentrations was used to the HepG2 cells

the medium glucose content was detected at different time points by the method of glucose-hexokinase and the HepG2 cell viability of different insulin concentrations was detected by the method of MTT

and to determine the optimal concentration and time of the insulin resistance;then the changes of cell morphology was observed by the oil red O staining;the expression of insulin receptor substance（IRS）-2 mRNA was detected by the method of real-time PCR and the expression of G-6-Pase was detected by the method of ELISA.Result: The best time of insulin resistance was 36 h

the best concentration of insulin resistance was 1×10-8 mol·L-1.After the establishment of the model

the lipid droplets of model cells was significantly increased compared with normal cells;the IRS-2 mRNA expression was significantly reduced compared with normal cells（P<0.05）;the G-6-Pase expression was significantly increased compared with normal cells（P<0.05）.Conclusion: By the method of insulin-induced in vitro

a stable HepG2 insulin resistance model can be established when the 1×10-8mol·L-1 concentration of insulin act on the HepG2 cell for 36 h.