均匀设计法优化丹参中4种活性成分的超声提取工艺

郜舒蕊; 赵志刚; 侯俊玲; 王文全; 朱俊杰; 王立会; 韩亚男

doi:10.13422/j.cnki.syfjx.2014100008

您当前的位置：

首页 >

文章列表页 >

均匀设计法优化丹参中4种活性成分的超声提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 均匀设计法优化丹参中4种活性成分的超声提取工艺
- Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design
- 中国实验方剂学杂志 2014年20卷第10期页码：8-12
- 作者机构：
- 作者简介：
- 基金信息：
  
  中药材规范化生产技术服务平台;国家工业与信息部中药材生产扶持项目（丹参规范化、规模化和产业化生产基地建设）
- DOI：10.13422/j.cnki.syfjx.2014100008
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
郜舒蕊, 赵志刚, 侯俊玲, 等. 均匀设计法优化丹参中4种活性成分的超声提取工艺[J]. 中国实验方剂学杂志, 2014,20(10):8-12.

GAO Shu-rui, ZHAO Zhi-gang, HOU Jun-ling, et al. Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 8-12.
郜舒蕊, 赵志刚, 侯俊玲, 等. 均匀设计法优化丹参中4种活性成分的超声提取工艺[J]. 中国实验方剂学杂志, 2014,20(10):8-12. DOI： 10.13422/j.cnki.syfjx.2014100008.

GAO Shu-rui, ZHAO Zhi-gang, HOU Jun-ling, et al. Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 8-12. DOI： 10.13422/j.cnki.syfjx.2014100008.

摘要

目的：优选丹参中迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA共4种活性成分的超声提取工艺。方法：以迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA提取量为指标，采用均匀设计考察乙醇体积分数、液料比、提取时间对丹参提取效率的影响，通过逐步非线性回归优选提取工艺条件。利用HPLC测定指标成分含量，流动相甲醇（A）-0.01%磷酸水（B）梯度洗脱（0~3 min，30%A；3~5 min，30%~40%A；5~13 min，40%A；13~20 min，40%~58%A；20~22 min，58%~75%A；22~24 min，75%A；24~50 min，75%~85%A），检测波长286 nm（迷迭香酸和丹酚酸B）和268 nm（隐丹参酮和丹参酮ⅡA）。结果：最佳超声提取工艺条件为乙醇体积分数75%，液料比200 mL·g-1，提取时间10 min；迷迭香酸、丹酚酸B、隐丹参酮及丹参酮ⅡA提取量分别为0.33，11.19，0.63，0.59 g·g-1，与2010年版《中国药典》中加热回流法相比，活性成分提取量无显著差异。结论：该超声提取工艺具有提取时间短、低毒、无污染等优点。

Abstract

Objective: To optimize ultrasonic extraction technology of rosmarinic acid

salvianolic acid B

cryptotanshinone and tanshinone ⅡA from Salviae Miltiorrhizae Radix et Rhizoma. Method: With extraction amounts of four active components as indexes

uniform design was adopted to optimize extraction technology by taking ethanol concentration

solid-liquid ratio

extracting time as factors.HPLC was employed to determine contents of four active components with mobile phase of methanol-0.01% phosphoric acid solution(0-3 min

30%A;3-5 min

30%-40%A;5-13 min

40%A;13-20 min

40%-58%A;20-22 min

58%-75%A;22-24 min

75%A;24-50 min

75%-85%A) for gradient elution and detection wavelengths at 286 nm(rosmarinic acid and salvianolic acid B) and 268 nm(cryptotanshinone and tanshinone ⅡA). Result: Optimum extraction conditions were as follows:ethanol concentration of 75%

liquid-solid ratio of 200 mL·g-1

extraction time 10 min;Under these conditions

extraction amounts of rosmarinic acid

salvianolic acid B

cryptotanshinone and tanshinone ⅡA were 0.33

11.19

0.63 and 0.59 g·g-1

respectively.Compared with heating reflux extraction in 2010 edition of "Chinese Pharmacopoeia"

Contents of four bioactive components had no significant differences. Conclusion: This optimum extraction process had advantages of low mammalian toxicity

solvent saving

fast extracting speed and so on.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

超高效液相色谱-质谱联用同时测定丹参中7种成分含量

2010年版《中国药典》一部含丹参制剂的提取工艺分析

根茎类药材丹参精准煮散饮片的质量评价

不同处理参数对丹参干制效率及活性成分含量的影响

大生产中丹参提取物制备工艺优选