基于均匀设计的白术总多糖提取工艺优选

盛云杰; 张永生

doi:10.13422/j.cnki.syfjx.2014100020

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基于均匀设计的白术总多糖提取工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 基于均匀设计的白术总多糖提取工艺优选
- Optimization of Extraction Technology of Total Polysaccharides from Atractylodis Macrocephalae Rhizoma Based on Uniform Design
- 中国实验方剂学杂志 2014年20卷第10期页码：20-22
- 作者机构：
- 作者简介：
- 基金信息：
  
  浙江省科技厅重点创新团队项目（2012R10044-03）;浙江中医药大学校级课题（2009ZY05）
- DOI：10.13422/j.cnki.syfjx.2014100020
  中图分类号： TQ461
- 纸质出版日期：2014
- 稿件说明：
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盛云杰, 张永生. 基于均匀设计的白术总多糖提取工艺优选[J]. 中国实验方剂学杂志, 2014,20(10):20-22.

SHENG Yun-jie, ZHANG Yong-sheng. Optimization of Extraction Technology of Total Polysaccharides from Atractylodis Macrocephalae Rhizoma Based on Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 20-22.
盛云杰, 张永生. 基于均匀设计的白术总多糖提取工艺优选[J]. 中国实验方剂学杂志, 2014,20(10):20-22. DOI： 10.13422/j.cnki.syfjx.2014100020.

SHENG Yun-jie, ZHANG Yong-sheng. Optimization of Extraction Technology of Total Polysaccharides from Atractylodis Macrocephalae Rhizoma Based on Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 20-22. DOI： 10.13422/j.cnki.syfjx.2014100020.

摘要

目的：优选白术总多糖的浸提工艺。方法：以总多糖质量分数为指标，采用均匀试验考察加水量、浸提时间和温度对提取工艺的影响。采用UV测定总多糖含量，检测波长491 nm。结果：最佳提取工艺参数为加8倍量水于90 ℃浸提60 min；白术水提液中总多糖质量分数6.20%。结论：优选的总多糖浸提工艺稳定可行，建立的含量测定方法准确可靠。

Abstract

Objective: To optimize extraction technology of total polysaccharides from Atractylodis Macrocephalae Rhizoma. Method: With the content of total polysaccharides as index

effects of the amount of water

extraction time and temperature on extraction technology were investigated by uniform design.UV was adopted to determine the content of total polysaccharides by detection wavelength was 491 nm. Result: Optimum extraction technology was as following:extracted 60 min with eight times the amount of water at 90 ℃;The content of total polysaccharides in water extract of Atractylodis Macrocephalae Rhizoma was 6.20%. Conclusion: Optimized extraction process was stable and feasible

this established determination was accurate and reliable.

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