中心组合设计-效应面法优化香青兰总黄酮磷脂复合物的制备工艺

胡宇飞; 刘宣麟; 何承辉; 黄伟; 邢建国

doi:10.13422/j.cnki.syfjx.2014100036

您当前的位置：

首页 >

文章列表页 >

中心组合设计-效应面法优化香青兰总黄酮磷脂复合物的制备工艺

工艺与制剂 | 更新时间：2024-01-04

- 中心组合设计-效应面法优化香青兰总黄酮磷脂复合物的制备工艺
- Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第10期页码：36-39
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区科技基础条件平台建设项目（PT1304）
- DOI：10.13422/j.cnki.syfjx.2014100036
  中图分类号： TQ461
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
胡宇飞, 刘宣麟, 何承辉, 等. 中心组合设计-效应面法优化香青兰总黄酮磷脂复合物的制备工艺[J]. 中国实验方剂学杂志, 2014,20(10):36-39.

HU Yu-fei, LIU Xuan-lin, HE Cheng-hui, et al. Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 36-39.
胡宇飞, 刘宣麟, 何承辉, 等. 中心组合设计-效应面法优化香青兰总黄酮磷脂复合物的制备工艺[J]. 中国实验方剂学杂志, 2014,20(10):36-39. DOI： 10.13422/j.cnki.syfjx.2014100036.

HU Yu-fei, LIU Xuan-lin, HE Cheng-hui, et al. Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 36-39. DOI： 10.13422/j.cnki.syfjx.2014100036.

摘要

目的：优选香青兰总黄酮磷脂复合物的制备工艺。方法：采用溶剂挥发法制备香青兰总黄酮磷脂复合物，通过单因素试验考察反应溶剂、反应时间、反应温度、香青兰总黄酮与大豆磷脂投料比、香青兰总黄酮质量浓度对复合率的影响。以香青兰总黄酮与大豆磷脂投料比、香青兰总黄酮质量浓度和反应时间为自变量，复合率为因变量，采用星点中心组合设计优选制备工艺，通过数学方程拟合，根据最佳数学模型描绘效应面，确定最佳处方工艺并进行验证试验。结果：最佳制备工艺条件为反应温度40 ℃，以甲醇-二氧六环（7∶3）混合溶液为反应溶剂，磷脂-香青兰总黄酮（2∶1），香青兰总黄酮质量浓度15 g·L-1，反应时间2 h；复合率实际值（96.42±1.58）%，与预测值96.18%的偏差<2%。结论：优选的制备工艺稳定可行，可用于工业生产制备。

Abstract

Objective: To optimize preparation process of total flavonoids from Dracocephalum moldevica-phospholipids complex. Method: Solvent evaporation method was adopted to prepare total flavonoids from D. moldevica-phospholipids complex.Based on single factor tests

with recombination rate as dependent variable

central composite design was adopted to optimize preparation technology by taking reaction time

ratio of total flavonoids to phospholipids and the concentration of total flavonoids as independent variables

fitting of various mathematical equations were performed using a statistical software of Design-Expert 8.0.6.Preparation parameters were optimized through response surface plotted by optimum fitting equations

optimized procedure was validated through experimental preparation of total flavonoids-phospholipids complex. Result: Optimum preparation technology was as following:reaction temperature 40 ℃

with methanol-dioxane(7:3) as a reaction solvent

ratio of phospholipids to total flavonoids of 2:1

total flavonoids concentration of 15 g·L-1 and reaction time 2 h;Under these conditions

recombination rate was up to (96.42±1.58)%

which had deviation of <2.0% between the observed value of recombination rate. Conclusion: Optimized preparation technology was feasible

reliable and suitable for industrial production of total flavonoids from D. moldevica-phospholipids complex.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

中心组合设计-效应面法优选化瘀祛痰颗粒的提取工艺

星点设计-效应面法优化粘胶分散型透皮贴剂的实验研究

各种实验设计方法在元胡止痛分散片处方优化中的应用研究

中心组合设计法优化黄连解毒黏附微球处方工艺

星点设计-效应面法优选丹参提取工艺