Objective: To determine the five anthraquinones in Rheum officinale and formulation by the method named quantitative analysis of multi-components by single marker(QAMS). Method: A HPLC-DAD method was developed to determine the contents of five anthraquinones

and QAMS methods were established when the five components were used as internal referring substances

respectively. The relative correction factors (RCFs) between the five anthraquinones (aloe-emodin

rhein

emodin

chrysophanol

physcion) were calculated and used to assay the contents of five anthraquinones in different batche R. officinale and formulations

and compared standards' calibration curves (SCCs) method to evaluate the accurary of QAMS method. The ratio of relative retention time was adopted to identify the accurate position of five anthraquinones. Result: When emodin as internal referring substance

the ranges of the relative error between QAMS and SCCs in different batches Rheum officinale and extrations and preparations were -3.91%-4.06%

-3.22%-3.27%

-5.39%-7.00%

respectively. The RSD of the relative retention value were 3.87%-9.92%. Conclusion: A rapid

accuracy

low-cost and convenient QAMS method can be used as a new quality assessment model for quanlity analysis of anthraquinones in Rheum officinale and formulation.