RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷

曲正义; 金银萍; 孙成贺; 李亚丽; 姚春林; 王英平

doi:10.13422/j.cnki.syfjx.2014100087

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RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷

化学与分析 | 更新时间：2024-01-04

- RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷
- Simultaneous Determination of Crenatoside and Acteoside in by RP-HPLC
- 中国实验方剂学杂志 2014年20卷第10期页码：87-89
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林市科技计划项目（201162512）
- DOI：10.13422/j.cnki.syfjx.2014100087
  中图分类号： R284
- 纸质出版日期：2014
- 稿件说明：
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曲正义, 金银萍, 孙成贺, 等. RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷[J]. 中国实验方剂学杂志, 2014,20(10):87-89.

QU Zheng-yi, JIN Yin-ping, SUN Cheng-he, et al. Simultaneous Determination of Crenatoside and Acteoside in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 87-89.
曲正义, 金银萍, 孙成贺, 等. RP-HPLC同时测定向日葵列当中苯乙醇苷类化合物crenatoside和类叶升麻苷[J]. 中国实验方剂学杂志, 2014,20(10):87-89. DOI： 10.13422/j.cnki.syfjx.2014100087.

QU Zheng-yi, JIN Yin-ping, SUN Cheng-he, et al. Simultaneous Determination of Crenatoside and Acteoside in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 87-89. DOI： 10.13422/j.cnki.syfjx.2014100087.

摘要

目的：建立同时测定向日葵列当中crenatoside和类叶升麻苷含量的HPLC法。方法：采用反相高效液相色谱法，用Agilent ZORBAX SB-C18 （4.6 mm×250 mm，5 μm）柱，以乙腈-0.01%冰醋酸溶液（18∶82）为流动相，流速1.0 mL·min-1，检测波长330 nm，柱温30 ℃，在30 min内分离检测了这两种化合物。结果：RP-HPLC测定的crenatoside和类叶升麻苷线性范围分别在在1.08~16.2 μg（r=0.999 9）和1.00~15.0 μg（r=0.999 8），平均加样回收率分别为97.23%，96.27%，RSD分别为1.21%，1.66%。结论：该方法简便，精密度、重复性良好，结果准确可靠，可以作为向日葵列当及其相关制剂的质量控制。

Abstract

Objective: To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides crenatoside and acteoside in Orobanche cumana. Method: The analysis was performed on a Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm) with CH3CN-0.01%HOAc(18:82) as mobile phase at a flow rate of 1.0 mL·min-1and at a column temperature of 30 ℃

330 nm as the detection wavelength

the well separation was achieved in 30 min. Result: Crenatoside showed good relationship at the range of 1.08-16.2 μg(r=0.999 9)

and acteoside at the range of 1.00-15.0 μg (r=0.999 8). The average recovery were 97.23% and 96.27%

relative standard derivation (RSD) of 1.21% and 1.66% for crenatoside and acteoside. Conclusion: The proposed method is simple

rapid

accurate and reliable.It can be used for the quality evaluation of O. cumana and its preparation.

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