乌头汤对大鼠的镇痛作用及初步机制探讨

王丹华; 刘春芳; 谭淑芳; 仲米存; 林娜

doi:10.13422/j.cnki.syfjx.2014100109

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乌头汤对大鼠的镇痛作用及初步机制探讨

药理 | 更新时间：2024-01-04

- 乌头汤对大鼠的镇痛作用及初步机制探讨
- Analgesic Effects and Mechanism of Wutou Decoction in Rats
- 中国实验方剂学杂志 2014年20卷第10期页码：109-112
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81173628，81374068）;国家重点基础研究发展计划（973）项目（2011CB505300，2011CB505305）
- DOI：10.13422/j.cnki.syfjx.2014100109
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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王丹华, 刘春芳, 谭淑芳, 等. 乌头汤对大鼠的镇痛作用及初步机制探讨[J]. 中国实验方剂学杂志, 2014,20(10):109-112.

WANG Dan-hua, LIU Chun-fang, TAN Shu-fang, et al. Analgesic Effects and Mechanism of Wutou Decoction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 109-112.
王丹华, 刘春芳, 谭淑芳, 等. 乌头汤对大鼠的镇痛作用及初步机制探讨[J]. 中国实验方剂学杂志, 2014,20(10):109-112. DOI： 10.13422/j.cnki.syfjx.2014100109.

WANG Dan-hua, LIU Chun-fang, TAN Shu-fang, et al. Analgesic Effects and Mechanism of Wutou Decoction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 109-112. DOI： 10.13422/j.cnki.syfjx.2014100109.

摘要

目的：研究乌头汤的镇痛作用及相关机制。方法：健康SD大鼠，随机分为正常组、模型组、乌头汤高、中、低剂量组以及布洛芬组，除正常组外其余各组建立慢性炎症痛模型，造模时在SD大鼠右踝关节腔部位注入完全弗氏佐剂（CFA）。乌头汤高、中、低剂量组于造模当天分别给予乌头汤4，2，1 g·kg-1，ig，布洛芬组30 mg·kg-1，ig，每日 1 次，连续 6 d。用游标卡尺测量大鼠患足踝关节肿胀度，冷板仪测量患足冷板痛阈值，爪触觉测试仪测量机械痛阈值，热测痛仪测量热辐射痛阈值，酶联免疫法检测血浆中前列腺素E2（PGE2）和5-羟色胺（5-HT）的水平，免疫组织化学检测背根神经节中瞬时感受器电位香草酸受体1（TRPV1）、瞬时感受器电位作用受体蛋白M8（TRPM8）的水平。结果：CFA致炎后，大鼠踝关节出现明显肿胀，冷板痛阈值、机械痛阈值和热辐射痛阈值明显降低；血浆中PGE2，5-HT含量明显升高；背根神经节中TRPV1的表达量明显升高，TRPM8的表达量明显降低。与模型组相比，乌头汤高、中剂量组能降低大鼠关节肿胀度（P<0.05），乌头汤各剂量组能升高冷板痛阈值（P<0.01或P<0.05）、高、中剂量组能提高机械痛阈值（P<0.01，P<0.05），高剂量组对热辐射痛阈值也有一定的增高作用（P<0.05），同时，能降低血浆中的PGE2，5-HT和背根神经节中的TRPV1表达水平，提高TRPM8表达水平。结论：乌头汤具有镇痛作用，其机制可能与降低血浆中PGE2和5-HT水平以及调节背根神经节中TRPV1和TRPM8的表达有关。

Abstract

Objective: To study the analgesic effects and mechanism of Wutou decoction. Method: Healthy SD male rats were randomly divided into normal group

model group

Wutou decoction high-dose group

Wutou decocotion middle dose group

Wutou decoction low-dose group and ibuprofen group. SD rats were injected with complete Freund's adjuvant (CFA) into the right ankle joint cavity to induce chronic inflammatory pain in joint. Wutou decoction at the doses of 4

1 g·kg-1

ibuprofen at the doses of 30 mg·kg-1 were daily ig administrated to rats from day 1 after the CFA injection for 6 days. Joint swelling

cold pain threshold

mechanical pain threshold and thermal radiation pain threshold of the diseased ankle joints were measured. The level of prostaglandin E2(PGE2)and 5-hydroxytryptamine (5-HT) in plasma were detected by enzyme-linked immunosorbant assay

and the levels of transient receptor potential vanilloid 1(TRPV1) and transient receptor potential melastatin 8(TRPM8) protein in dorsal root ganglion (DRG) measured by immunohistochemistry. Result: The ankle joints of rats in model group were obviously swollen

and the cold pain threshold

mechanical pain threshold and thermal radiation pain threshold were significantly decreased compared to the rats in normal group. The levels of PGE2 and 5-HT in plasma and the expression level of TRPV1 in DRG were significantly increased too

and production of TRPM8 in DRG is decreased. Compared with the model group

Wutou decoction high-dose group and middle dose group reduced the joint swelling (P<0.05)

all dose group significantly increased cold pain threshold (P<0.01 or P<0.05)

high-dose group and middle dose group increased mechanical pain threshold (P<0.01

P<0.05)

and high-dose group reduced thermal radiation pain threshold (P<0.05). The levels of PGE2

5-HT and TRPV1 are decreased while the level of TRPM8 is increased after the treatment of Wutou decoction. Conclusion: Wutou decoction has the analgesic effect

and the mechanism may be related to inhibiting the level of PGE2 and 5-HT in plasma and regulating the expression of TRPV1 and TRPM8 in DRG.

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