五味子提取液抑菌活性研究

闫绍悦; 林树乾; 傅剑; 李桂明; 王岱杰; 万仁忠

doi:10.13422/j.cnki.syfjx.2014100142

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五味子提取液抑菌活性研究

药理 | 更新时间：2024-01-04

- 五味子提取液抑菌活性研究
- Evaluation of Bacteriostatic Activity of Extract from Schisandrae Chinensis Fructus
- 中国实验方剂学杂志 2014年20卷第10期页码：142-146
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家农业部公益性行业（农业）科研专项项目（201303040-10）;山东省自然科学基金项目（ZR2012CQ042）
- DOI：10.13422/j.cnki.syfjx.2014100142
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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闫绍悦, 林树乾, 傅剑, 等. 五味子提取液抑菌活性研究[J]. 中国实验方剂学杂志, 2014,20(10):142-146.

YAN Shao-yue, LIN Shu-qian, FU Jian, et al. Evaluation of Bacteriostatic Activity of Extract from Schisandrae Chinensis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 142-146.
闫绍悦, 林树乾, 傅剑, 等. 五味子提取液抑菌活性研究[J]. 中国实验方剂学杂志, 2014,20(10):142-146. DOI： 10.13422/j.cnki.syfjx.2014100142.

YAN Shao-yue, LIN Shu-qian, FU Jian, et al. Evaluation of Bacteriostatic Activity of Extract from Schisandrae Chinensis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 142-146. DOI： 10.13422/j.cnki.syfjx.2014100142.

摘要

目的：探究五味子提取液的抑菌活性。方法：采用水提醇沉法提取五味子活性成分，以大肠埃希菌、枯草芽孢杆菌、沙门菌、金黄色葡萄球菌4种标准菌株和环丙沙星耐药及敏感的大肠杆菌野株为供试菌，以环丙沙星为对照药物，采用牛津杯法测定供试菌分别培养16，32，48，64 h后五味子提取液的抑菌圈直径，监测五味子提取液的抑菌活性和抑菌活性的变化趋势，同时采用试管双倍稀释法测定五味子提取液对大肠杆菌耐药株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果：五味子提取液对各供试菌的抑菌圈直径在20.16~30.06 mm，五味子提取液对环丙沙星耐药大肠埃希菌的最低抑菌质量浓度在0.0156 3~0.062 5 g·mL-1，最低杀菌质量浓度在0.312 5~0.062 5 g·mL-1。五味子提取液对所有供试菌都有明显的抑制作用，与环丙沙星相比，五味子提取液对环丙沙星耐药菌的抑制作用表现出极大的优势。环丙沙星与五味子提取液对供试菌的抑菌活性都呈逐渐下降趋势。结论：五味子提取液不仅具有广谱抗菌活性，对环丙沙星耐药菌也具有显著的抑制活性，可开发为新型抗菌剂。

Abstract

Objective: To investigate the bacteriostatic activity of water extracting-alcohol precipitating solution of Schisandrae Chinensis Fructus. Method: Extracting the active compounds by the method of water extracting-alcohol precipitating

with reference culture strains including Escherichia coli

Bacillus subtilis

Staphylococcus aureus

Salmonella and wild E.coli as tested bacteria

the antimicrobial activity variation tendency of the extract of Schisandrae Chinensis Fructus and ciprofloxacin at incubation time 16

64 h was monitored by the method of cup plate assay method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by two-fold dilution method. Result: Extract of Schisandrae Chinensis Fructus had a notable inhibiting microorganism activity to all tested bacteria. Extract of Schisandrae Chinensis Fructus exhibit great superiority compared to ciprofloxacin in inhibiting the ciprofloxacin-resistant strains. The antimicrobial activity variation of Schisandrae Chinensis Fructus and ciprofloxacin showed a gradual decline tendency. Conclusion: The study indicates that extract of Schisandrae Chinese Fructus could be a new antibacterial agent due to its broad-spectrum bacteriostatic activity and the inhibiting activity to resistant bacteria.

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