Objective: To investigate the effects of Keluoxin capsule on hypoxia-induced angiogenesis in retina. Method: In vitro

cultured ARPE-19 was pretread with 5

10

20 mg·L-1 Keluoxin for 24 hours

before 150 μmol·L-1 CoCl2 treatment for 24 hours

ELISA and Western blotting were used to detected the level of vascular endothelia growth factor(VEGF) and hypoxia inducible factor 1 alpha(HIF1-α)

expressions of VEGF and HIF1-α mRNA were measured by real-time PCR. In vivo

sixty C57BL/6J mice were randomly divided into six groups as follows:control group

retinopathy(OIR) model group

calcium dobesilate group and Keluoxin groups. In control group

mice were raised in regular air environments. The fifty mice were fed under the environment with 75%±2%oxygen for five days and then returned to normal air to establish OIR model. The 0.5 g·kg-1 calcium dobesilate was administered by gavage feeding once daily in calcium dobesilate group

and 0.5

1.0

2.0 g·kg-1 Keluoxin were gavaged as low

middle and high dosage Keluoxin groups respectively. Five days later

the mice were sacrificed using excessive anesthesia method and the retinal sections were prepared for the calculation of the number of endothelial cell nuclei broken retinal inner membrane after hemotoxylin and eosin staining. The morphologic changes of retinal vessels were observed after ADPase staining. Result: The VEGF secretion

mRNA expression of VEGF and HIF1-α of CoCl2 group were significantly higher than control group(P<0.001)

and those were decreased by 10

20 mg·L-1 Keluoxin remarkably (P<0.01 and P<0.05). In vivo

there was a obvious reduction in expansion

deformation and A value of vascular in Keluoxin groups compared with OIR model group

and the number of cell nuclei broken the inner limiting membrane had decreased in Keluoxin groups(P<0.01) significantly. Conclusion: Keluoxin capsule can inhibit hypoxia-induced VEGF secretion and neovascularization in retina.