Objective: To investigate the effect of low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) on Dectin-1

Syk and CARD9 mRNA expression of dendritic cells. Method: Dendritic cells derived from bone marrow of ICR mice were cultured in vitro; the expression of CD11c and CD86 molecule on the surface of dendritic cells was detected by flow cytometry; the effect of LMPAB on the proliferation of T lymphocytes stimulated by dendritic cells was detected by WST. Real-time quantitative PCR detect Dectin-1 mRNA

Sky mRNA and CARD9 mRNA expression levels. Result: CD11c and CD86 protein expression were expressed by 98.6% and 96.97% respectively; compared with the control group (4.84±0.21)

LMPAB+OVA group (6.59±0.19) enhances the effect of dendritic cells on stimulating T lymphocytes' proliferation (P<0.05). Compared with the mRNA expression of Dectin-1 (6.13±0.27)

Sky (0.36±0.11) and CARD9(1.85±0.20) in the control group

Dectin-1 (8.25±0.19)

Sky (1.63±0.16) and CARD9 (4.40±0.19) mRNA expression in LMPAB+OVA group was significantly increased with statistically significant (P<0.05). Conclusion: The cells derived from bone marrow were dendritic cells; immunizing potency could be enhanced by LMPAB regulating and controlling Dectin-1

Sky

CARD9 mRNA expression levels in Dendritic cells.