猪皮胶原肽的分离纯化及结构鉴定

宋景; 刘长龙; 高鹏; 代龙

doi:10.13422/j.cnki.syfjx.2014110023

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猪皮胶原肽的分离纯化及结构鉴定

工艺与制剂 | 更新时间：2024-01-04

- 猪皮胶原肽的分离纯化及结构鉴定
- Separation and Indentification of Collagen Peptides from Pigskin
- 中国实验方剂学杂志 2014年20卷第11期页码：23-25
- 作者机构：
- 作者简介：
- 基金信息：
  
  山东省自然科学基金项目（ZR2011HM051）;国家“十一五”科技支撑计划项目（2008BAI53B073）
- DOI：10.13422/j.cnki.syfjx.2014110023
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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宋景, 刘长龙, 高鹏, 等. 猪皮胶原肽的分离纯化及结构鉴定[J]. 中国实验方剂学杂志, 2014,20(11):23-25.

SONG Jing, LIU Chang-long, GAO Peng, et al. Separation and Indentification of Collagen Peptides from Pigskin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 23-25.
宋景, 刘长龙, 高鹏, 等. 猪皮胶原肽的分离纯化及结构鉴定[J]. 中国实验方剂学杂志, 2014,20(11):23-25. DOI： 10.13422/j.cnki.syfjx.2014110023.

SONG Jing, LIU Chang-long, GAO Peng, et al. Separation and Indentification of Collagen Peptides from Pigskin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 23-25. DOI： 10.13422/j.cnki.syfjx.2014110023.

摘要

目的：分离纯化猪皮胶原肽并鉴定其活性成分的相对分子质量分布及结构。方法：采用胃蛋白酶和胰蛋白酶酶解猪皮，酶解液超滤，电渗析除盐，冻干得猪皮胶原肽，利用Sephadex-G25凝胶柱色谱对酶解产物进行分离纯化，分析各部位对L929细胞增值作用的影响，运用ESI-Q-TOF2质谱仪对活性部位进行肽序列分析。结果：分离纯化得到2个活性部位（部位Ⅰ，Ⅱ），二者对L929细胞的增殖率分别为162%，179%。活性部位Ⅱ相对分子质量主要分布于800~2 000 Da，首次发现GLGAGGGRGSDGNPG，PAGAHGPAGL共2个序列。结论：猪皮胶原肽具有显著的细胞增殖作用，符合G-X-Y胶原排列组合，具有一定专属性。

Abstract

Objective:To isolate and purity collagen peptides from pigskin

then identify relative molecular mass distribution and structure of its active ingredients. Method:Pigskin was digested by pepsin and trypsin

enzyme solution received ultrafiltration

electrodialysis desalination and lyophilization to get collagen peptides from pigskin

Sephadex G-25 gel column was adopted to separate and purity hydrolyzate

effects of each part of collagen peptides on L929 cell multiplication were analyzed

ESI-Q-TOF2 mass spectrometer was employed to analyze amino acid sequence of collagen peptides from pigskin. Result:Two parts were separated as part Ⅰ and Ⅱ

both of them on L929 cell multiplication rates were 162% and 179%

respectively.Relative molecular mass of activity part Ⅱ were 800-2 000 Da

two amino acid sequence were first discovered

they were GLGAGGGRGSDGNPG and PAGAHGPAGL. Conclusion:Collagen peptides from pigskin had a significant cell proliferation with some specificity

which met permutations of G-X-Y.

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