Objective:To optimize purification technology of lobster sauce streptokinase by macroporous resin. Method:Type of macroporous resin was screened by taking moisture adsorption as index;With specific activity of lobster sauce streptokinase as index

single factor tests were adopted to optimize purification process parameters

such as adsorption performance(loading quantity of sample

adsorption time) and elution performance(species

pH and volume of eluent

elution rate)

purification effect was analyzed by SDS-PAGE electrophoresis. Result:HPD-400 macroporous resin had a good adsorbability to lobster sauce streptokinase

optimum purification conditions were as follows:sample volume of 2 BV

adsorption time of 2 h

removed impurities with 2 BV of water

eluted with 4 BV of Tris HCl-30% ethanol buffer(pH 8.0) with adsorption speed of 2 BV·h-1;under these conditions

specific activity of lobster sauce streptokinase was 2 254.29 U·mg-1

purification multiple was 44.17 times

we could receive 2.86 mg of protein in 20 mL of fermentation liquor

lobster sauce streptokinase showed a single strip in electrophoretogram whose relative molecular weight was 28 kD. Conclusion:HPD-400 macroporous resin could be adopted to purify lobster sauce streptokinase

this optimized purification technology was stable and feasible.