YANG Xiu-mei, WANG Jin, HUANG Qin-wan, et al. Simultaneous Quantitative Analysis of Three Flavonoids in Pericarpium Citri Reticulatae by Multi-components Assay by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 45-49.
YANG Xiu-mei, WANG Jin, HUANG Qin-wan, et al. Simultaneous Quantitative Analysis of Three Flavonoids in Pericarpium Citri Reticulatae by Multi-components Assay by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 45-49. DOI: 10.13422/j.cnki.syfjx.2014110045.
Objective:To establish a method for determination of hesperidin
nobiletin and tangeretin in Pericarpium Citri Reticulatae. And to verify the accuracy and feasibility of the method. Method:Using hesperidin as standard substance
the relative correction factor of the other two flavonoids were determined by HPLC. Using the external standard method and the relative correction factor calculated the content of the three flavonoids
then comparing the results. Result:The results of external standard method and quantitative analysis of multi-components by one marker (QAMS) have no significant difference. Conclusion:Determination of hesperidin
nobiletin and tangeretin by QAMS is a accurate and feasible method.