SUN Dong-mei, TAN Zhi-can, BI Xiao-li, et al. Determination of Extracts in Microctis Folium by PLS-UV Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 62-65.
SUN Dong-mei, TAN Zhi-can, BI Xiao-li, et al. Determination of Extracts in Microctis Folium by PLS-UV Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 62-65. DOI: 10.13422/j.cnki.syfjx.2014110062.
Objective:To develop a novel method for fast determination of extracts content in Microctis Folium with PLS-UV Spectrophotometry. Method:Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method. Result:The correlation coefficient (R2) of the calibration models was 0.964 1
and the prediction average relative deviation for extracts content were 1.73%. Conclusion:PLS-UV technique used in this study is timesaving and accuracy. It is qualified to rapidly analyze the extracts content of traditional Chinese medicine whose components are complex. PLS-UV can be used to determine the extracts content of Microctis Folium.