Objective:To establish the methods for determination of total polysaccharides and rutin content in Alhagi sparsifolin

and to compare the content of each sample from the different parts. Method:With glucose as control

total polysaccharides were measured by UV spectrophotometry at detection wavelength of 620 nm by anthrone sulphuric acid colorimetry. The content of rutin was determined by HPLC. The determination was performed on Symmetry shield Rp18 (3.9 mm×150 mm

5 μm) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solvent (19:81) with the detection wavelength at 360 nm and the column temperature at 30 ℃. Result:The linear range of total polysaccharides was 3.85-70.33 mg·L-1(r=0.995 9)

and the linear range of rutin was 4.95-59.4 mg·L-1(r=0.999 6). Total polysaccharides and rutin in A. sparsifolin from different parts had certain differences. Conclusion:A. sparsifolin cultivated in the fruit and in part on the ground harvest in the total polysaccharides and the content of rutin are higher. It will provide foundation for the research for A. sparsifolin cultivation and harvest.