阳桃根中苯醌类化合物的分离鉴定与含量测定

温庆伟; 陈春霞; 梁杏梅; 徐小惠; 黄仁彬

doi:10.13422/j.cnki.syfjx.2014110070

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阳桃根中苯醌类化合物的分离鉴定与含量测定

化学与分析 | 更新时间：2024-01-04

- 阳桃根中苯醌类化合物的分离鉴定与含量测定
- Isolation, Identification and Determination for Benzoquinone Compounds from Root
- 中国实验方剂学杂志 2014年20卷第11期页码：70-74
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81160533，81360129）
- DOI：10.13422/j.cnki.syfjx.2014110070
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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温庆伟, 陈春霞, 梁杏梅, 等. 阳桃根中苯醌类化合物的分离鉴定与含量测定[J]. 中国实验方剂学杂志, 2014,20(11):70-74.

WEN Qing-wei, CHEN Chun-xia, LIANG Xing-mei, et al. Isolation, Identification and Determination for Benzoquinone Compounds from Root[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 70-74.
温庆伟, 陈春霞, 梁杏梅, 等. 阳桃根中苯醌类化合物的分离鉴定与含量测定[J]. 中国实验方剂学杂志, 2014,20(11):70-74. DOI： 10.13422/j.cnki.syfjx.2014110070.

WEN Qing-wei, CHEN Chun-xia, LIANG Xing-mei, et al. Isolation, Identification and Determination for Benzoquinone Compounds from Root[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 70-74. DOI： 10.13422/j.cnki.syfjx.2014110070.

摘要

目的：研究阳桃根（ACLR）中苯醌类化学成分的类型及含量测定方法。方法：利用色谱分离法、核磁共振技术对阳桃根药材60%乙醇提取物中的环己烷萃取部位进行分离、鉴定，并应用HPLC法对药材中的苯醌类化学成分进行含量测定。结果：分离得到2个苯醌类化合物：2-methoxy-6-nonyl-cyclohexa-2，5-diene-1，4-dione （MNDD）；2-dodecyl-6-methoxy-cyclohexa-2，5-diene-1，4-dione （DMDD）；MNDD，DMDD分别在0.177~2.657 μg和0.094~1.416 μg进样量与峰面积有良好的线性关系；平均加样回收率为98.96%（RSD 1.59%），99.55%（RSD 0.98%）。结论：首次从植物中分离得到2个苯醌类化合物；HPLC测定方法简便、快速、稳定、准确可靠、重复性好，可用于阳桃根药材中MNDD，DMDD的质量控制。

Abstract

Objective:To isolate and identify the benzoquinone compounds from Averrhoa carambola root (ACLR) and establish its HPLC determination method. Method:The cyclohexane fraction of 60% ethanol extract of the herb was separated by the repeated silica gel column

and identified by 1H-NMR

13C-NMR

HSQC

HMBC and FTIR

etc. And the determination method of benzoquinone compound isolated from ACLR was established by HPLC. Result:Two benzoquinone compounds were isolated from ACLR

namely 2-methoxy-6-nonyl-cyclohexa-2

5-diene-1

4-dione (MNDD)

2-dodecyl-6-methoxy-cyclohexa-2

5-diene-1

4-dione (DMDD). MNDD and DMDD had a good linear relationship between sample size and peak area at the range of 0.177-2.657 μg and 0.094-1.416 μg

and their average recovery were 98.96% (RSD 1.14%) and 99.55% (RSD 0.98%)

respectively. Conclusion:The two benzoquinone compounds were separated for the first time from plants. And the HPLC determination method can used for ACLR quality control for its convenience

rapidity

stability

accuracy

reliability and good reproducibility.

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