Objective:To investigate the inhibitory effect of corn silk extract and anthocyanins on Escherichia coli

Staphylococcus aureus and Klebsiella pneumoniae. Method:The turbidimetric method (600 nm) was used for determining bacterial growth of E. coli(1.6×106cfu/mL)

S. aureus (5.6×106cfu/mL) and K. pneumoniae(1.8×106cfu/mL) by adding corn silk extract(0

35

70

140 g·L-1)

anthocyanin(0.312

0.625

1.25 g·L-1) and corn silk extract(70 g·L-1)-anthocyanin(0.625 g·L-1)mixed solutions for 6 h. Result:The results showed that a time and dose dependent inhibitory curve of corn silk extract and anthocyanins on three bacteria were observed within 6 h. The inhibitory rates of corn silk extract (70 g·L-1) and anthocyanins (0.625 g·L-1) were (31.3±2.42)% and (21.6±1.89)% on E. coli

(25.3±2.85)% and (37.2±1.74)% on S. aureus

and (39.7±1.34)% and (15.3±3.67)% on K. pneumoniae

respectively

while the effect of their combination was (76.8±2.53)% on E. coli

(80.1±1.74)% on S. aureus and (67.3±1.53)% on K. pneumoniae. Conclusion:The compatibility of corn silk extract and anthocyanins can not only expand the antibacterial spectrum

and also has synergistic effects on pathogenic bacteria to prevent and treat chronic urinary tract infection.