Objective:This study is to observe the effect of Tongmai Dixian pills on the angiogenesis of ischemic myocardium border zone and to research myocardial protection mechanisms. Method:One hundred male rats with chronic myocardial ischemia model except the sham group

which were randomized

model group

Tongmai Dixian pills high

middle and low (6.3

3.15

1.57 g·kg-1) dose groups

and Shexiang Baoxin pills(0.014 2 g·kg-1) group. Intragastric administration for six weeks

hematoxylin-eosin (HE) staining of myocardial pathomorphology changes and immunohistochemical staining observed neovascularization of myocardial infarct border zone. Result:①Tongmai Dixian pills can deduce the scope of myocardial infarctions as to protect the heart.②Tongmai Dixian pills on the myocardial tissue (HE staining):sham group's the cardiac muscle fibers arranged in neat rows and inflammatory cells infiltration; model group's cardiocytes had serious edema

lots of disorganized myocardial interstitial inflammatory cells infiltration

or even visible its focal necrosis; Tongmai Dixian pills each dose group myocardial nucleus size is uniform

showing varying degrees of fibroblasts and inflammatory cells infiltration; Shexiang Baoxin pills group under the light microscopic is similar to Tongmai Dixian pills middle dose group; ③ischemic myocardial angiogenesis microvessel density(MVD):compared with the sham group

model group with significant difference (P<0.05)

Tongmai Dixian pills each dose group can be significantly increased(P<0.05 or P<0.01)

Tongmai Dixian pills high

middle dose and Shexiang Baoxin pills can also be significantly increased (P<0.01)

Tongmai Dixian pills low dose group is also an increasing trend

but no statistically significant differences compared with model group. Conclusion:Tongmai Dixian pills can promote the generation microvascular in the myocardial infarction border zone in order to achieve the purpose of protecting the myocardium.