超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选

郭涛; 孙莉; 黄艳; 吕光耀; 张丙云

doi:10.13422/j.cnki.syfjx.2014130008

您当前的位置：

首页 >

文章列表页 >

超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选
- Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System
- 中国实验方剂学杂志 2014年20卷第13期页码：8-11
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81360476）;甘肃自然科学基金项目（1112RJZA016）
- DOI：10.13422/j.cnki.syfjx.2014130008
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
郭涛, 孙莉, 黄艳, 等. 超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):8-11.

GUO Tao, SUN Li, HUANG Yan, et al. Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 8-11.
郭涛, 孙莉, 黄艳, 等. 超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):8-11. DOI： 10.13422/j.cnki.syfjx.2014130008.

GUO Tao, SUN Li, HUANG Yan, et al. Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 8-11. DOI： 10.13422/j.cnki.syfjx.2014130008.

摘要

目的：优选超声波辅助双水相体系提取竹叶椒总生物碱的工艺条件。方法：采用超声波辅助丙醇-硫酸铵双水相体系提取竹叶椒总生物碱，选择提取时间、醇水质量比和盐用量为自变量，总生物碱提取率为因变量，通过三因素三水平的响应面试验优选提取工艺。结果：最佳提取工艺为提取时间40 min，醇水质量比0.5：1，盐用量7.79 g；总生物碱提取率1.704%，与模型预测值（1.711%）偏差很小，纯度19.8%。结论：超声波辅助双水相体系法提取总生物碱具有效率高、纯度高的特点，可避免不稳定或易变性的生物活性分子变性。

Abstract

Objective: To optimize ultrasonic assisted aqueous two-phase system extraction technology of total alkaloids from Zanthoxylum armatum. Method: Total alkaloids from Z. armatum was extracted by ultrasonic extraction technology coupling with propyl-alcohol ammonium sulfate aqueous two-phase system

taking extracting time

mass ratio of alcohol to water and salt dosage as independent variables

extraction rate of total alkaloids as dependent variable

a three response factors and three levels composite design of response surface methodology was employed to optimize extraction technology. Result: Optimal extraction process was as following:extracting time of 40 min

mass ratio of alcohol to water 0.5:1

salt dosage 7.79 g.Under these conditions

extraction rate of total alkaloids was up to 1.704%

which was agreed with model predicted value(1.711%)

purity of total alkaloids was 19.8%. Conclusion: Ultrasound assisted aqueous two-phase system for total alkaloids from Z. armatum had advantages of high extraction rate and high purity

which could avoid denaturation of bioactive molecules with instability or volatility.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

南天竹总生物碱对三氧化二砷减毒作用及其LC-MS分析

小檗皮总生物碱提取物的大孔树脂纯化工艺与质量标准考察

咳喘平片提取工艺的实验研究

荷叶中生物碱提取及纯化工艺研究