女贞子连续超声逆流提取工艺优选

张静; 陈红专; 董立彬; 王海亮

doi:10.13422/j.cnki.syfjx.2014130016

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女贞子连续超声逆流提取工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 女贞子连续超声逆流提取工艺优选
- Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus
- 中国实验方剂学杂志 2014年20卷第13期页码：16-18
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项（2011ZX09401-306）
- DOI：10.13422/j.cnki.syfjx.2014130016
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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张静, 陈红专, 董立彬, 等. 女贞子连续超声逆流提取工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):16-18.

ZHANG Jing, CHEN Hong-zhuan, DONG Li-bin, et al. Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 16-18.
张静, 陈红专, 董立彬, 等. 女贞子连续超声逆流提取工艺优选[J]. 中国实验方剂学杂志, 2014,20(13):16-18. DOI： 10.13422/j.cnki.syfjx.2014130016.

ZHANG Jing, CHEN Hong-zhuan, DONG Li-bin, et al. Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 16-18. DOI： 10.13422/j.cnki.syfjx.2014130016.

摘要

目的：优选女贞子的连续超声逆流提取工艺。方法：以齐墩果酸和熊果酸总量为指标，通过正交试验考察超声频率、乙醇体积分数、料液比和温度对提取工艺的影响。采用HPLC测定齐墩果酸和熊果酸含量，流速0.33 mL·min-1，流动相乙腈-甲醇-0.5%冰醋酸溶液（19：67：14）。结果：最佳提取工艺为加15倍量80%乙醇于30 kHz提取；齐墩果酸和熊果酸平均总质量分数5.66%，RSD 1.64%。结论：优选的连续超声逆流提取工艺操作简单、科学合理，适合于女贞子工业化大生产。

Abstract

Objective: To optimize successive ultrasound countercurrent extraction process of Ligustri Lucidi Fructus. Method: Taking total amount of oleanolic acid and ursolic acid as index

orthogonal design was adopted to investigate effects of ethanol concentration

ultrasonic frequency

solid-liquid ratio and temperature on extraction process of Ligustri Lucidi Fructus.ELSD was employed to determine contents of oleanolic acid and ursolic acid with mobile phase of acetonitrile-methanol-0.5% acetic acid(19:67:14) and flow rate of 0.33 mL·min-1. Result: Optimum extraction process was that extracted with 15 times the amount of 80% ethanol at 30 kHz; under these conditions

average total amount of oleanolic acid and ursolic acid was 5.66% with RSD of 1.64%. Conclusion: This optimized extraction technology was reasonable and scientific

which was suitable for large scale production of Ligustri Lucidi Fructus.

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