多指标综合评分法优选制何首乌的-环糊精辅助提取工艺

崔明磊; 任晓亮; 刘亚男; 邱喜龙; 戚爱棣

doi:10.13422/j.cnki.syfjx.2014130018

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多指标综合评分法优选制何首乌的-环糊精辅助提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 多指标综合评分法优选制何首乌的-环糊精辅助提取工艺
- Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method
- 中国实验方剂学杂志 2014年20卷第13期页码：18-21
- 作者机构：
- 作者简介：
- 基金信息：
  
  高等学校博士学科点专项科研基金项目（20111210110010，20111210120006）
- DOI：10.13422/j.cnki.syfjx.2014130018
  中图分类号： R284.2
- 纸质出版日期：2014
- 稿件说明：
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崔明磊, 任晓亮, 刘亚男, 等. 多指标综合评分法优选制何首乌的-环糊精辅助提取工艺[J]. 中国实验方剂学杂志, 2014,20(13):18-21.

CUI Ming-lei, REN Xiao-liang, LIU Ya-nan, et al. Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 18-21.
崔明磊, 任晓亮, 刘亚男, 等. 多指标综合评分法优选制何首乌的-环糊精辅助提取工艺[J]. 中国实验方剂学杂志, 2014,20(13):18-21. DOI： 10.13422/j.cnki.syfjx.2014130018.

CUI Ming-lei, REN Xiao-liang, LIU Ya-nan, et al. Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 18-21. DOI： 10.13422/j.cnki.syfjx.2014130018.

摘要

目的：优选制何首乌的β-环糊精（β-CD）辅助提取工艺。方法：采用层次分析法，以二苯乙烯苷、大黄素及大黄素甲醚转移率的综合评分为指标，通过正交试验考察β-CD用量、料液比、提取次数及时间对制首乌有效成分提取工艺的影响。采用HPLC测定二苯乙烯苷、大黄素及大黄素甲醚含量，流动相乙腈（A）-0.1%磷酸水（B）梯度洗脱（0~5 min，15%~25%A；5~12 min，25%~30%A；12~15 min，30%~60%A；15~20 min，60%A；20~25 min，60%~75%A；25~31 min，75%~77%A），检测波长320（二苯乙烯苷），254 nm（大黄素、大黄素甲醚）。结果：最佳工艺条件为β-CD加入量10%，加10倍量水提取3次，每次1 h；二苯乙烯苷、大黄素及大黄素甲醚转移率分别为90.19%，8.39%，0.76%。结论：与传统水提法相比，β-CD辅助提取法能显著提高活性成分转移率。

Abstract

Objective: To optimize β-cyclodextrin(β-CD) assisted extraction process of Polygoni Multiflori Radix Praeparata. Method: Analytic hierarchy process was adopted

taking comprehensive score of transfer rates of emodin

physcion and stilbene glycoside as index

orthogonal test was applied to optimize extraction technology with solid-liquid ratio

β-CD dosage

extracting time and times as factors.HPLC was employed to determine contents of emodin

physcion and stilbene glycoside with mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-5 min

15%-25%A;5-12 min

25%-30%A;12-15 min

30%-60%A;15-20 min

60%A;20-25 min

60%-75%A;25-31 min

75%-77%A) and detection wavelength at 320(stilbene glycoside) and 254(emodin and physcion) nm. Result: Optimal extraction process was as following:extracted 3 times with 10-fold water for 1 h each time

β-CD dosage of 10%;Transfer rates of emodin

physcion and stilbene glycoside were 8.39%

0.76% and 90.19%

respectively. Conclusion: Compared with traditional water extraction

β-CD assisted extraction process could significantly improve transfer rate of active ingredients.

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